大豆黄酮对衰老小鼠脑组织SOD、LDH同工酶的影响  

Effects of Daidzein on SOD Isoenzymes and LDH Isoenzymes in the Brain of Aged Mice

作　　者：刘文[1] 毛缜[1] 陈龙[2] 沈露露[1] 程超[1] 郑元林[1] 

机构地区：[1]徐州师范大学生命科学学院江苏省药用植物生物技术重点实验室,徐州221116 [2]南京师范大学江苏省资源生物技术重点实验室,南京210008

出　　处：《动物学杂志》2004年第5期19-24,共6页Chinese Journal of Zoology

基　　金：教育部高等学校骨干教师资助计划项目 ;江苏省重点实验室开放基金资助项目 (No.KJS0 2 0 2 4 ) ;江苏省教育厅自然科学基金资助项目 (No.98KJB180 0 0 3)

摘　　要：利用SOD和LDH同工酶电泳分析 ,研究大豆黄酮对衰老小鼠的抗氧化作用。结果显示大豆黄酮没有改变SOD和LDH同工酶谱的特征 ,但对因衰老引起的小鼠脑组织LDH和SOD同工酶活性、各组分的相对活性和比活力的变化有不同程度的改善作用 ,即LDH同工酶中LDH 2、LDH 3的活性明显下降 ,LDH 1的活性下降最为明显 ,而LDH 4的活性有所下降 ,但不显著 ,LDH 5的活性几乎没有变化 ,SOD同工酶的SOD 1和SOD 2的活性有不同程度的升高。这表明大豆黄酮是通过抑制LDH同工酶H亚基的合成来降低LDH的活性 ,而对M亚基的合成没有影响 ,并且能够促进SOD同工酶SOD 1和SOD 2的合成 ,不影响其遗传稳定性。In order to study the antioxidant effects of daidzein,we analyzed the SOD isoenzymes and LDH isoenzymes in the brain of aged mice by polyacylamide gel electrophoresis (PAGE).The results showed that daidzein did not affect the isozyme-spectra of SOD and LDH, but improved to varying degrees the activities of SOD isoenzymes and LDH isoenzymes, as well as the relative activities and specific activities of different subunits The most evident decrease was observed in the activities of LDH-1 and LDH-2. LDH-3 activity decreased remarkably, LDH-4 activity showed a little decrease, while nearly no change was observed in LDH-5 activity. The activities of SOD-1 and SOD-2 increased to different degrees. The results show that daidzein reduces the activity of LDH by inhibiting the synthesis of H subunit, ,but promotes the synthesis of SOD-1 and SOD-2 without affecting the stability of inherence.

关 键 词：LDH同工酶 活性 SOD-1 脑组织 衰老小鼠 影响 改善作用 大豆黄酮 SOD同工酶 亚基 

分 类 号：Q41[生物学—生理学]