肿瘤-睾丸-脑抗原家族新成员PNMA5的克隆和表达谱分析  

Cloning and Identification of PNMA5, a Novel Member of Cancer-Testis-Brain Antigen Family

作　　者：董学员[1] 杨小昂[1] 苏艳蓉[1] 李云燕[1] 乔欢[1] 尹艳慧[1] 陈慰峰[1] 

机构地区：[1]北京大学医学部免疫学系,北京100083

出　　处：《中国生物化学与分子生物学报》2004年第5期587-591,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家 973自然科学基金资助项目 (No .G19990 5 3 90 4);北京市自然科学基金资助项目 (No .70 0 10 0 2 )~~

摘　　要：综合运用几种生物信息学数据库 ,分析了人类X染色体上可能的未知基因 ,筛选获得一个新的肿瘤抗原基因并克隆鉴定了它的全长cDNA .该基因全长为 3194bp ,编码一个 4 48个氨基酸残基的蛋白质 ,它与肿瘤 睾丸 脑抗原家族成员PNMA1、PNMA2及PNMA3有很高的同源性 ,命名为PNMA5 .在 16种人正常组织中 ,PNMA5仅在睾丸与脑中表达 ;而在所检测的肝癌、胃癌、肠癌、肺癌、乳腺癌与头颈部肿瘤 ,PNMA5在肠癌中表达 .PNMA5是肿瘤 睾丸 脑抗原家族的一个新成员 .认识这些肿瘤 睾丸 脑抗原的意义在于它们在肿瘤组织的表达可能是肿瘤破坏机体免疫耐受的一种机制 ,其相关神经副肿瘤综合症可能是一些隐匿肿瘤的早期表现 .All the potential unknown genes in the X chromosome were analyzed with a bioinformatics in which several databases were used. A novel tumor antigen encoding gene was obtained. The full-length cDNA is 3194bp encoding a protein of 448 amino acid residues. The tumor antigen shares homology with PNMA1, PNMA2 and PNMA3, members of cancer-testis-brain antigen family and was consequently named PNMA5. The mRNA expression of PNMA5 was determined in 16 human normal tissues and different histological types of tumors. The results demonstrated that PNMA5 was expressed in colon cancer and normal testis and brain. PNMA5 is a novel member of the cancer-testis-brain antigen family. The expression of the cancer-testis-brain antigens in tumors may be a mechanism that tumors break the patients′ immune tolerance resulted in paraneoplastic neurological syndrome.

关 键 词：生物信息学 X染色体 肿瘤-睾丸-脑抗原 

分 类 号：Q786[生物学—分子生物学]