新型H5N1亚型禽流感灭活疫苗对鸭、鹅及鸽免疫原性研究  

Immunogenicity evaluation of an inactivated H5N1 subtype avian influenza vaccine in ducks, geese and pigeons

作　　者：张苏华[1] 刘佩红[1] 周锦萍[1] 潘水春 袁明龙 曹火仁 刘康明 徐新红[1] 田国彬[6] 

机构地区：[1]上海市畜牧兽医站,上海200000 [2]上海市南汇区畜牧兽医站,上海200000 [3]上海市青浦区畜牧兽医站,上海200000 [4]上海市松江区畜牧兽医站,上海200000 [5]上海市嘉定区畜牧兽医站,上海200000 [6]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2004年第6期401-404,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：上海科技攻关计划项目(农科攻字2004第12_1号);国家"十五"科技攻关计划项目(2000BA519A)资助

摘　　要：本研究就反基因操作分子修饰致弱种毒株制备新型H5N1亚型禽流感灭活疫苗对鸭、鹅和鸽子的免疫原性进行了系统评估。结果表明该疫苗对水禽及鸽子具有良好免疫原性。根据实验结果,初步推荐该禽流感灭活疫苗对上述禽类的免疫程序,即鸭:2周龄0 5mL皮下注射,3月龄1 0mL肌肉注射二免,9月龄1 0mL肌肉注射三免;鹅:10日龄0 5mL皮下注射,3～4周龄1 5mL肌肉注射加强免疫,4月龄1 5mL进行第3次免疫;鸽子:2周龄以0 3mL首免,4周龄以0 5mL加强免疫,6月龄以0 5mL第3次免疫。The immunogenicity of an inactivated H5N1 subtype avian influenza vaccine generated from a molecular modified attenuated reassortant seed virus was evaluated in ducks,geese and pigeons,and results demonstrated that the vaccine induced strong hemagglutinination inhibition antibody immune response in waterfowls and pigeons.Based on the results,we propose the vaccination procedures as following: ducks could be vaccinated with the first dose of 0.5?mL at the age of 2_week_old and the second dose of 1.0?mL at the age of 3_month_old,and a third dose of 1.0?mL could be applied at the age of 9_month_old; geese could be vaccinated with the first dose of 0.5?mL at the age of 10_day_old and the second dose of 1.5?mL at the age of 4_week_old,and a third dose of 1.5?mL could be applied at the age of 4_month_old;pigeons could be vaccinated with the first dose of 0.3?mL at the age of 2_week_old and the second dose of 0.5?mL at the age of 4_week_old,and a third dose of 0.5?mL could be applied at the age of 6_month_old.

关 键 词：月龄 周龄 禽流感 H5N1亚型 鸽子 灭活疫苗 日龄 肌肉注射 免疫原性研究 加强免疫 

分 类 号：S858[农业科学—临床兽医学] S855.3[农业科学—兽医学]