用链球菌自制荧光抗体检测猪链球菌的研究  

Study on diagnosis S.suis infection with self_preparation immunofluorescence antibody

作　　者：于新和[1] 陶海静[1] 杜桂欣 刘景超 

机构地区：[1]郑州牧业工程高等专科学校,河南郑州450008 [2]河南省淅川县成人中专,河南淅川474200 [3]开封县畜牧局,河南开封475000

出　　处：《中国预防兽医学报》2004年第6期462-464,共3页Chinese Journal of Preventive Veterinary Medicine

摘　　要：为达到利用荧光抗体建立一种诊断猪链球菌病的有效方法的目的。先提取兔抗猪链球菌IgG ,用FITC标记并进一步用G5 0过滤 ,制备出兔抗猪链球菌荧光抗体。同时利用吸收试验、特异性抑制试验对自制的荧光抗体进行了检验。结果表明利用自制荧光抗体进行链球菌病的诊断可在 2～ 3h内对链球菌作出诊断 ,比直接镜检更具有特异性和敏感性。试验中发现人工感染小白鼠 2 0h后 ,小鼠荧光抗体染色均呈阳性 ,其中以血液及肺脏中荧光强度最强 ;链球菌荧光抗体染色与葡萄球菌有一定的交叉 。To establish an effective method for diagnosis S.suis infection.We picked up rabbit anti_S.suis IgG,Marked with FITC,filtrated with G_50 adn then we acquired rabbit anti_S.suis immunofluorescence antibody.The immunofluorescence antibody was examined by absorb experiment and specific inhibit experiment.The result showed that by using immunofluorescence antibody diagnosis S.suis infection has higher Specific and Sensitive than direct microscopic examination and can draw a conclusion within two hour.In this experiment we also find that all viscera of Mouse have positive reaction 20 hour after injection with S.suis and the blood and lung have the highest positive reaction.The rabbit anti_S.suis immunofluorescence antibody has a cross reaction with staphylococcus and not with other common pathogen bacterium.

关 键 词：猪链球菌病 荧光标记兔抗猪链球菌抗体 诊断 

分 类 号：S852.611[农业科学—基础兽医学]