聚乙二醇对血红蛋白离子交换层析行为的影响  

Effects of Polyethylene Glycol on the Ion Exchange Chromatographic Behaviors of Hemoglobin

作　　者：赵东旭[1] 路秀玲[2] 苏志国[2] 

机构地区：[1]北京理工大学生命科学与技术学院,北京100081 [2]中科院过程工程研究所生化工程国家重点实验室,北京100080

出　　处：《北京理工大学学报》2004年第10期924-928,共5页Transactions of Beijing Institute of Technology

基　　金：国家自然科学基金资助项目(20136020)

摘　　要：研究作为共溶剂的聚乙二醇(PEG600和PEG4000)在离子交换层析法纯化血红蛋白过程中对血红蛋白层析行为的影响.预处理后的牛血红细胞破裂液用QSepharoseBigBeads阴离子交换介质进行层析.层析过程中添加质量分数为0.5%～5.0%的PEG600或PEG4000,血红蛋白回收率由76%提高到90%以上,血红蛋白纯度略有增加并且其生物活性得到较好地保持,PEG600对血红蛋白层析的保留时间影响不大,PEG4000使血红蛋白的层析保留时间增加,增加强度与PEG的浓度之间呈正相关,并用共溶剂理论对上述结果进行了讨论.Effects of polyethylene glycol (PEG600 and PEG4000) on the ion exchange chromatographic behaviors of bovine hemoglobin (Hb) was studied. Pretreated bovine hemolysate was chromatographied with Q Sepharose Big Beads, and 0.5%～5.0% PEG600 or PEG4000 was added as a cosolvent. Consequently, the purity of Hb increased slightly, near to electrophoretical purity. The recovery of Hb was obviously higher than the control (76%), especially in the presence of PEG4000, which was over 90%. Bioactivity of Hb was well preserved in the process of chromatography after adding the cosolvent.The retention time of Hb in chromatography was not affected when PEG600 was added as the cosolvent, while it was longer in the presence of PEG4000 and the time increased as the concentration of PEG4000 improved. The results were discussed based on the theory of cosolvent.

关 键 词：血红蛋白 聚乙二醇 离子交换层析 共溶剂 

分 类 号：O657.75[理学—分析化学]