蛇床子素和纳米氧化锆强韧化磷酸钙支架对成骨细胞增殖影响的研究  

The study on the effect of the factors of osthole and hyper-porosity Ca_3 (PO4)_2 bone cells scaffold/NM ZrO_2 reinforced on osteoblast

作　　者：吕宏[1] 施杞[1] 王拥军[1] 李家顺[2] 叶晓健[2] 张巧艳[3] 蔡舒[4] 张立德[5] 

机构地区：[1]上海中医药大学附属龙华医院伤骨科,上海200032 [2]第二军医大学附属长征医院骨科 [3]第二军医大学药学院 [4]天津大学材料学院 [5]中科院固体所

出　　处：《脊柱外科杂志》2004年第5期280-284,共5页Journal of Spinal Surgery

基　　金：国家自然科学基金资助项目 ( 3 0 170 95 4)

摘　　要：目的　研究蛇床子素与纳米氧化锆强韧化磷酸钙骨支架的相容性 ,以及其在体外对幼兔成骨细胞增殖和成骨作用的影响。方法　酶消化法分离培养幼兔股骨成骨细胞 ,噻唑兰法 (MTT)测量成骨细胞增殖率 ,用碱性磷酸酶试剂盒测定细胞内骨碱性磷酸酶 (ALP)活性。结果　蛇床子素对大鼠新生成骨细胞的增殖和ALP活性具有显著的促进作用 ,与纳米氧化锆强韧化磷酸钙人工骨支架生物相容性良好。结论　纳米氧化锆强韧化磷酸钙人工骨支架复合蛇床子素刺激的成骨细胞 。Objective To study the compatibility and the effect of osthole and hyper-porosity calcium phosphate [Ca_3(PO4)_2]bone cells scaffold/nanometer(NM) zirconium oxide (ZrO_2) reinforced on osteoblast.Methods Osteoblasts were isolated from infant rabbit thighbone by trypsin and collagenase digestion. The rate of osteoblast proliferation was measured by MTT. The activity of alkaline phosphatase (ALP) was measured by using ALP reagent box. Results Osthole obviously promoted osteoblast proliferation and activity of ALP in the rat, and had a good bio compatibility with hyper-porosity Ca_3 (PO4)_2 bone cells scaffold/NM ZrO_2.ConclusionOsteoblast reinforced by osthole and hyper-porosity Ca_3 (PO4)_2 bone cells scaffold/NM ZrO_2 is an optimal compounded material for bone tissue engineering.

关 键 词：成骨细胞 蛇床子素 骨支架 增殖 磷酸钙人工骨 ALP 酶试剂 骨碱性磷酸酶 影响 体外 

分 类 号：R318[医药卫生—生物医学工程] R285[医药卫生—基础医学]