金雀异黄素抑制氯化钴诱导的人视网膜色素上皮细胞缺氧诱导因子1α表达的研究  

Genistein inhibited hypoxia inducible factor-1α expression induced by cobalt chloride in human retinal pigment epithelium cells

作　　者：李华[1] 严虹[1] 潘金顺[1] 王斌[1] 

机构地区：[1]南京医科大学药理与神经科学系,江苏南京210029

出　　处：《医学研究生学报》2004年第11期973-975,979,共4页Journal of Medical Postgraduates

基　　金：国家自然科学青年基金资助项目 (批准号 :3 0 0 0 0 2 0 7) ;江苏省教育厅重点项目 (批准号 :KJB3 10 0 0 1) ;江苏省高校高新技术产业化项目 (批准号 :JH0 1 0 5 0 ) ;南京医科大学创新基金资助项目 (CX2 0 0 10 0 3 )

摘　　要：目的 :研究氯化钴 (CoCl2 )诱导体外培养的人视网膜色素上皮 (RPE)细胞缺氧诱导因子 1α(HIF 1α)的表达以及金雀异黄素 (Gen)对其表达的影响。　方法 :采用免疫组化和激光共聚焦显微镜研究HIF 1α的表达。　结果 :CoCl2 可明显诱导人RPE细胞HIF 1α的表达 ,0 .5h达高峰 ,随后逐渐下降。Gen可呈浓度依赖性地抑制氯化钴诱导的HIF 1α蛋白表达。　结论 :Gen可抑制CoCl2 诱导的人RPE细胞HIFObjective:The time-dependent changes of hypoxia-inducible factor-1α (HIF-1α) expression induced by CoCl_(2 ) treatment and the effects of genistein on the level of HIF-1α expression in human retinal pigment epithelium cells were studied. Methods:Using a confocal scanning laser microscope coupled to a computer, HIF-1α expression was determined. Results:CoCl_(2 ) treatment could significantly elevate the level of HIF-1α expression. At 0.5 hour after CoCl_(2 ) treatment, the highest level was observed. Genistein 50 μmol/L, 100 μmol/L, 200 μmol/L could suppress HIF-1α expression in a concentration-dependent manner. Conclusions:These results suggested that genistein could inhibit HIF-1α expression induced by CoCl_(2).

关 键 词：金雀异黄素 缺氧诱导因子1Α 氯化钴 人视网膜色素上皮细胞 

分 类 号：R329.6[医药卫生—人体解剖和组织胚胎学]