与桃果实非酸/酸性状连锁AFLP特异片段的克隆及序列分析  

Cloning and Sequence Analysis of AFLP Specific Fragment Related to Fruit Non-acid/acid Trait of Peach

作　　者：吴俊[1,2] 束怀瑞[3] 张学宁[4] 张开春[4] 姜立杰[4] 

机构地区：[1]山东农业大学园艺学院 [2]南京农业大学园艺学院,江苏南京210095 [3]农业大学园艺学院 [4]北京市农林科学院林业果树研究所

出　　处：《果树学报》2004年第6期526-529,共4页Journal of Fruit Science

基　　金：北京科技合同项目(954830300);北京市新星项目(953812200)。

摘　　要：利用AFLP技术从京玉、美味正反交F1代69株群体中筛选到3个特异扩增片段,与桃果实非酸/酸性状紧密连锁。回收特异片段并将其克隆、测序,结果表明特异片段A、B、C均来自于EcoRI与MseI两内切酶酶切片段,大小依次为140、199、408bp,利用序列分析软件推测片段B、C中可能含有与糖水平调控作用相关的因子。上述结果对AFLP标记向SCAR标记的转换具有重要意义。Three specific amplified DNA fragments linked to fruit non-acid /acid trait of peach were obtained from 69 F1 seedlings of the intra-specific cross between cultivars 'Jingyu' and 'Flavortop' by using AFLP technique. After cloning the specific fragments and sequencing,we found that fragment A,B and C all were digested by both EcoRⅠand MseⅠ,and were 140 bp,199 bp,and 408 bp in length respectively. It was presumed that fragments B and C might contain elements which are relevant to regulating the sugar content by utilizing sequence analysis software. This study would be important for further SCAR marker conversion.

关 键 词：桃 非酸/酸 AFLP 克隆 序列 

分 类 号：S662.1[农业科学—果树学]