成熟猕猴桃果实中乙烯受体基因Ad-ETR2的克隆与序列分析  

Cloning and Sequence Analysis of An Ethylene Receptor Fragment (Ad-ETR2) From Ripening Kiwifruit

作　　者：魏绍冲[1] 陈昆松[1] 

机构地区：[1]浙江大学果实分子生理与生物技术实验室/农业部园艺植物生长发育与生物技术重点开放实验室,浙江杭州310029

出　　处：《果树学报》2004年第6期544-547,共4页Journal of Fruit Science

基　　金：国家重点基础研究发展规划项目973项目G2000046806;国家自然科学基金资助项目(30170660);高等学校博士学科点专项科研基金项目资助(20040335022)。

摘　　要：在分析GenBank登录的植物乙烯受体家族氨基酸和核苷酸保守区序列基础上,设计简并引物,通过RT-PCR扩增出1个657bp大小的cDNA片段,测序结果表明,该片段穴Ad-ETR2雪编码219个氨基酸,它与其它植物乙烯受体核苷酸同源性为79.9%～86.5%,氨基酸的同源性为85.4%～95.9%,序列分析推断该基因片段可能是与番茄Le-ETR1结构和功能类似的一类乙烯受体。A pair of degenerated primers was designed according to the conserved sequences of plant ethylene receptor genes,and a 657 bp fragment was amplified by RT- PCR. The results suggested that this fragment,referring to as Ad-ETR2, encoded 219 amino acids. Ad-ETR2 shared 79.9%～80% and 85.4%～95.9% similarity with the ethylene receptors from other plants at nucleotide and polypeptide level,respectively. This fragment seemed to be a ethylene receptor similar to tomato Le-ETR1 based on sequence analysis.

关 键 词：猕猴桃 乙烯受体 果实成熟 序列分析 

分 类 号：S663.4[农业科学—果树学]