西瓜品种和育种材料的DNA指纹分析  

DNA Fingerprinting Analysis of Varieties and Inbreds in Watermelon

作　　者：刘立功[1] 许勇[1] 张雪平[1] 张仁兵[2] 张海英[1] 屈广琪[1] 陈雅萍[3] 

机构地区：[1]国家蔬菜工程技术研究中心,北京100089 [2]浙江大学园艺系,浙江杭州310029 [3]首都师范大学生物系,北京100089

出　　处：《果树学报》2004年第6期573-578,共6页Journal of Fruit Science

基　　金：863计划项目2001AA241174;北京市自然科学基金重点项目5991017。

摘　　要：利用RAPD技术对国内外26份西瓜材料进行遗传亲缘关系研究、构建每份材料的特异指纹。在720个RAPD随机引物中筛选出15个引物用于聚类分析,将供试26份材料分为6个类群1个美国生态型类群、2个中间生态型类群、1个东亚生态型类群、2个野生类群。将京欣一号杂种纯度鉴定的RAPD特异标记K14-1500和N04-600成功转化为SCAR标记,并应用于京欣一号纯度鉴定实践。RAPD analysis was applied to assess the genetic relationships among 26 accessions of watermelon [Citrullus lanatus(Thunb.) Mansf.], and to construct fingerprinting of these accessions. Results of clustering analysis based on polymorphism amplified with the fifteen primers which were screened from 720 arbitrary 10-mer primers showed that the 26 accessions were assigned to 6 groups which were 1 East-Asia group, 1 American group, 2 medial groups and 2 African wild groups. RAPD makers, K14-1 500 bp and N04-600 bp were successfully transformed to SCAR markers, and were used for identification of the purity of watermelon hybrid Jingxin №.1.

关 键 词：西瓜(Citrullus lanatus) RAPD SCAR DNA指纹 纯度鉴定 

分 类 号：S651[农业科学—果树学]