高压氧治疗STZ大鼠糖尿病性心肌病变作用机理的研究  

Mechanism of High Pressure Oxygen in Treatment of Diabetic Heart Disease in STZ Rats

作　　者：周亚刚 刘芳 张杰 邵贵强 周景花 彭铭淑 钟慈声[6] 施劲东 张高峰 伍旭升 许澎 

机构地区：[1]上海市第五人民医院心内科 [2]上海市第五人民医院内分泌科 [3]上海市第五人民医院病理科 [4]上海市第五人民医院高压氧科 [5]上海市第五人民医院免疫室 [6]复旦大学上海医学院电镜室,上海200240

出　　处：《中国临床医学》2004年第4期542-544,共3页Chinese Journal of Clinical Medicine

摘　　要：目的 :检测经高压氧治疗 (HBOT)的有糖尿病性心肌病变的STZ(链脲佐菌素 )大鼠血浆细胞因子水平 ,探讨高压氧治疗糖尿病性心肌病变的作用机理。方法 :STZ糖尿病大鼠造模成功 3个月、电镜证实有糖尿病性心肌病变后 ,随机分为三组 :(1)HBOT组 (n =30 ) :每天接受 0 .15MPa、HBOT 1h ,连续 2 0d。 (2 )糖尿病对照组 (n =32 ) :不做治疗。 (3)正常对照组 (n =2 0 ) :腹腔注射生理盐水的无糖尿病的正常大鼠。分别在疗程 5d、10d、2 0d后处死三组大鼠 ,测定三组大鼠血清NGF、IGF - 1、TGF - β、NO和MMP - 1水平。结果 :HBO治疗 10d后 ,HBOT组心肌病变明显好于同期糖尿病对照组 ,低于正常对照组 ;血清NGF、NO水平稍有增高 ,但与对照组间无显著差异。HBOT 2 0d后 ,血清NGF和NO水平显著高于糖尿病对照组 (均P <0 .0 1) ,治疗组血IGF - 1水平显著高于DM组 (P =0 .0 0 0 84 ) ,但仍低于正常对照组 (P =0 .0 0 15 3) ;血清MMP - 1、TGF - β水平较糖尿病组明显降低 (P <0 .0 5 )。结论 :HBOT改善STZ大鼠糖尿病性心肌病变的机理可能与促进NGF、IGF - 1合成和血清NO增加、抑制MMP- 1和TGF -Objective: To study the mechanism.of hyperbaric oxygen therapy(HBOT) in diabetic cardiomyopathy by inestigating the serum levels of cytokine in STZ-induced diabetic rats. Methods: Diabetes with diabetic cardiomyopathy was induced in 62 SD rats by single intraperitoneal injection of 1.0% STZ. The HBOT group (n=30) was treated with HBOT (0.15MPa for 1 h) for 20 days and insulin. The diabetic control group (n=32) was treated by only insulin. The normal control group (n=20) was treated with normal saline injected into abdominal cavity. The myocardial pathogenesis and expression of TGF -β were detected at day 5,10 and 20.The serum levels of IGF-1 NGF, TGF-β, NO and MMP-1were determined in 3 group after 5 days, 10 days, and 20 days of receiving HBOT, respectively. Results: After 10 days of receiving HBOT, the diabetic cardiomyopathy was significently improved in HBOT rats compared with diabetic controls and healthy controls; and serum NGF, NO levels were higher slightly, but no significant difference between HBOT group and controls. After 20 days of receiving HBOT, serum NGF, NO and IGF-1 levels in HOBT rats were higher than diabetic controls (P<0.01, P<0.01, P= 0.000849, respectively), but IGF-1 levels were lower than health controls (P=0.00153); Serum MMP-1, TGF-β levels were significantly lower than diabetic controls (P<0.05). Conclusion:We conclude that the mechanism of HBOT’s treating diabetic cardiomyopathy in STZ-induced diabetic rats was related to stimulating synthesis of NGF and IGF-1, elevating serum NO, and depressing MMP-1 and TGF-β.

关 键 词：高压氧治疗 STZ大鼠 糖尿病性心肌病变 细胞因子 

分 类 号：R542.2[医药卫生—心血管疾病] R587.2[医药卫生—内科学]