去势兔骨质疏松动物血清TRAP及BALP水平的观察  

Serun levels of tartrate-resistant acid phophatase and bone alkaline phosphatase in ovariectomized rabbits with osteoporosis

作　　者：蔡桂英[1] 侯静[1] 杨霞[1] 魏玲[1] 

机构地区：[1]华西医科大学生物化学教研室

出　　处：《中国骨质疏松杂志》1997年第4期38-41,共4页Chinese Journal of Osteoporosis

摘　　要：破骨细胞生成的抗滴石酸盐酸性磷酸酶（ｔａｒｔｒａｔｅ－ｒｅｓｉｓｔａｎｔａｃｉｄｐｈｏｓｐｈａｔａｓｅ，ＴＲＡＰ）及成骨细胞生成的骨碱性磷酸酶（ｂｏｎｅａｌｋａｌｉｎｅｐｈｏｓｐｈａｔａｓｅ，ＢＡＬＰ）均可分泌入血。因此，血中ＴＲＡＰ及ＢＡＬＰ水平可分别反映破骨细胞及成骨细胞的功能。本文在以往工作的基础上，观察了经双侧卵巢切除法诱导五月龄新西兰纯种大白兔形成骨质疏松动物的血清ＴＲＡＰ（采用对硝基酚磷酸盐法）及ＢＡＬＰ（热失活法）水平，结果显示：术后兔血清ＴＲＡＰ及ＢＡＬＰ活性均较术前升高，ＴＲＡＰ的变化先于ＢＡＬＰ。术后１月ＴＲＡＰ活性升至最高，然后逐渐降低，至术后２．２月左右基本降至术前水平；ＢＡＬＰ活性在术后２．２月左右升至最高，并在此水平上保持约半个月，其后很快下降，于术后３．７月左右基本降至手术前水平。对照兔血清ＴＲＡＰ及ＢＡＬＰ活性的变化趋势基本一致。结果说明：手术去势后首先刺激破骨细胞的骨吸收作用，然后刺激成骨细胞的骨形成作用。这可能是女性绝经后头几年内骨质快速丢失的一个原因。有关骨质疏松动物模型血中ＴＲＡＰ及ＢＡＩ．Ｐ变化规律的研究，目前国内外尚未见报道。本文研究结果为原发性骨质疏松的病因学、The serum levels of tartrate-resistant acid phosphatase (TRAP)and bone alkaline phosphatase (BALP)were asssayed dynamically in ovariectomized rabbits with osteoporosis by the methods of p-nitrophenyl phosphate and heat-inactivation,respectively. The results indicated that the postoperational serum levels of both TRAP and BALP in bilaterally ovariectomized rabbits were higher than those prior to operation,but the change in TRAP was earlier than that in BALP. The serum level of TRAP reached highest at one month after ovariectomy,then decreased gradually and returned to the preoperational level at about 2.2 months after operation and kept unchanged in half a month, then decreased rapidly and returned to the preoperational level at 3.7 months after operation. The change tendencies of seruml evels of TRAP and BALP in the control group were basically consistent. The results suggest that the change of TRAP as a sign of bone resorption occurs earlier than that of BALP as a sign of bone formation in serum of ovariectomized rabbits.

关 键 词：BALP TRAP 血清 骨质疏松 术后 破骨细胞 成骨细胞 观察 左右 刺激 

分 类 号：R681[医药卫生—骨科学] R723[医药卫生—外科学]