南极冰藻Chlorophyceae L-4抗氧化酶活性对UV-B辐射增强的响应  

Response of Antioxidase Activity in Antarctic Ice Microalgae Chlorophyceae L-4 to UV-B Radiation Enhancement

作　　者：缪锦来[1] 王波[2] 阚光锋[2] 姜英辉[2] 侯旭光[3] 李光友[1] 

机构地区：[1]国家海洋局第一海洋研究所 [2]中国海洋大学生命学院,山东青岛266003 [3]山东大学生命学院,山东济南250100

出　　处：《海洋科学进展》2004年第3期313-319,共7页Advances in Marine Science

基　　金：国家自然科学基金资助项目--南极冰藻中抗紫外辐射活性物质的研究(40206022)

摘　　要：为了了解在UV B辐射增强的胁迫下,南极冰藻ChlorophyceaeL 4细胞内保护酶系统对活性氧自由基的清除作用,对不同辐射强度下ChlorophyceaeL 4细胞中丙二醛含量的变化、自由基的产生速率和相应的保护酶系统的变化进行了测定。结果表明,在UV B辐射增强的初期(1～3d),ChlorophyceaeL 4中丙二醛含量的迅速提高,但随后很快降低,在低强度UV B(35μW·cm-2)辐照下保护酶活性能够恢复到基本水平。在UV B辐射增强的胁迫下,ChlorophyceaeL 4中的超氧化物歧化酶、过氧化物酶和过氧化氢酶的活性明显比对照组的高,而且在高强度(70μW·cm-2)的辐照下,酶活性更高。ChlorophyceaeL 4中的抗坏血酸过氧化物酶的活性在UV B辐射增强的胁迫下略有升高或变化不大。本研究结果表明,保护酶系统在南极冰藻适应南极强辐射环境中起着非常重要的作用。In order to understand the active oxygen free radical clearing function of protecting enzymes in antarctic ice mircoralgae Chlorophyceae L-4 at diferent UV-B radiation intensities, the malonaldehyde(MAD) content changes, the free radical generation rate and the corresponding protecting enzyme changes in Chlorophyceae L-4 at Different UV-B radiation intensities were determined. It is shown from the determined results that the MAD content in Chlorophyceae L-4 increased rapidly in early days (1 to 3 days) of UV-B radiation enhancement, then decreased rapidly, and the protecting enzyme activity could return to fundamental level at low UV-B radiation intensity (35μm·cm^(-2)). Under the stress of UV-B radiation enhancement, the superoxide dismutase(SOD), peroxidase (POD) and catalase (CAT) activities in Chlorophyceae L-4 were obviously higher than those in the control, and their activities became higher at high UV-B radiation intensity (70μm·cm^(-2)). The ascorbic acid peroxidase (APX) activity in Chlorophyceae L-4 increased slightly or kept stable under the stress of UV-B radiation enhancement. The above experimental results indicate that the protecting enzyme system plays an important role in the adaptation of antarctic ice microalgae to the strong antarctic radiation environment.

关 键 词：南极冰藻 绿藻 UV-B辐射 酶 响应 

分 类 号：Q949.2[生物学—植物学]