细胞因子对关节软骨细胞增殖的实验研究  

The Effects of Cytokines on the Proliferation of Joint Chondrocyte

作　　者：谈志龙[1] 邢国胜[1] 李德达[1] 王淑云[1] 于顺禄[1] 白人骁[1] 李士民[1] 张凯[1] 王毅[1] 

机构地区：[1]天津医院骨科研究所,天津市300211

出　　处：《骨与关节损伤杂志》2004年第9期614-616,共3页The Journal of Bone and Joint Injury

摘　　要：目的　探讨细胞因子对关节软骨细胞增殖的影响。方法　应用关节软骨细胞体外培养 ,分别加入IL - 1β,TNF -α,TGF - β1,IL - 1β和TGF - β1,TNF - β和TGF - β1,采用MTT法观察软骨细胞增殖情况 ,瑞氏染色观察细胞形态及分裂指数测定。结果　细胞因子作用软骨细胞 2 4h ,与对照组比较 ,1ng/ml、 10ng/mlTGF - β1组 ,10ng/mlIL - 1β和 1ng/mlTGF - β1组 ,高于对照组。细胞因子作用软骨细胞 4 8h ,与对照组比较 ,2 0ng/mlIL - 1β组 ,1ng/ml、 10ng/mlTNF -α组 ,0 1、 1、 10ng/mlTGF - β1,10ng/mlIL - 1β和 1ng/mlTGF - β1组 ,1ng/mlTNF -α和 1ng/mlTGF - β1组 ,高于对照组。IL - 1β,TNF -α作用软骨细胞 2 4h ,形态发生改变。培养 4 8h ,分裂指数测定 10ng/mlTGF - β1组显著高于对照组。结论　TGF - β1对体外培养关节软骨细胞有明显促进细胞分裂与增殖作用 ,IL - 1β ,TNF -α,作用软骨细胞 4 8h也有促进细胞增殖作用。Objective To study the effects of cytokine on the proliferation of joint chondrocyte. Methods Joint chondrocyte was cultured in vitro, IL-1β, TNF-α,TGF-β 1 were added into the culture medium respectively. Cell proliferation was measured by MTT cell morphology and mitotic index was investigated by using wright staining. Results Almost in all groups treated with cytokines, the OD values were higher than that in the control group, including: 24 hours after cytokines treated, TGF-β 1 at 1ng/ml, IL-1β at 10ng/ml & TGF-β 1 at 1ng/ml, TGF-β 1 at 10ng/ml; after 48 hours treated, IL-1β at 20ng/ml, TNF-α at 1ng/ml、10ng/ml, TGF-β 1 at 0.1、1、10ng/ml, IL-1β at 10ng/ml & TGF-β 1 at 1ng/ml, TNF-α at 1ng/ml & TGF-β 1 at 1ng/ml. The appearance of chondrocyte was changed after 24-hour IL-1β, TNF-α treated.The mitotic index in TGF-β 1 at 10ng/ml was higher than that in control.Conclusion TGF-β 1 may increase the division and proliferation of joint chondrocyte in vitro pronouncedly, joint chondrocyte proliferation in vitro also increased after 48 hours with IL-1β, TNF-α treated.

关 键 词：TGF-Β1 IL-1Β 对照组 关节软骨细胞 TNF-α 细胞因子 增殖 形态发生 细胞分裂 体外培养 

分 类 号：R684[医药卫生—骨科学] R735[医药卫生—外科学]