MCI-186对高糖环境中受损人真皮微血管内皮细胞的保护作用  

Effect of MCI-186 on glucose induced cellular injury of cultured human dermal microvascular endothelial cells

作　　者：董叫云[1] 青春[1] 郭静苹[1] 陆树良[1] 高見佳宏 田中秀治[2] 山口亮 尾乡贤 

机构地区：[1]上海第二医科大学瑞金医院灼伤整形科上海市烧伤研究所,上海200025 [2]日本杏林大学医学院形成外科&热伤中心

出　　处：《上海第二医科大学学报》2004年第9期703-706,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家重点基础研究发展规划项目("973"项目)(G1999054205)资助.

摘　　要：目的 观察高糖环境对人真皮微血管内皮细胞(HDMEC)功能的影响,探讨清除自由基对高糖所致HDMEC损伤的保护作用。方法 建立高糖环境下HDMEC培养的体外模型,并加入自由基清除剂MCI-186予以干扰,同时检测细胞的增殖、形态、黏附等生物学行为。结果 高糖环境可影响内皮细胞的增殖,改变其特异性形态,增加H2O2诱导的细胞毒性。MCI-186可缓解高糖所致的内皮细胞增殖受抑,降低内皮细胞表面黏附性,下调高糖诱导的细胞凋亡。结论 控制血糖的同时清除有害自由基,可能对糖尿病合并难愈创面的治疗起到积极作用。Objective To investigate the role of a free radical scavenger in preventing endothelial cell damages induced in high glucose condition. Methods The high level of glucose in human dermal micro vascular endothelial cell (HDMEC) culture model was established in vitro with or without MCI-186. Changes of cellular proliferation, morphology and leukocyte-adhesion were detected, and cell apoptosis was observed through DNA fragment and caspase-3 activity. Results High glucose condition decreased endothelial cell proliferation and enhanced H2O2-induced leukocyte adhesion to endothelial cells. It also increased cell apoptosis. Among those cellular damages of HDMEC with high glucose condition, the cellular proliferation, leukocyte adhesion and cell apoptosis were partially improved by MCI-186 treatment. Conclusion These results suggest that under well control of glycemia the scavenging free radical might contribute to the therapy of impaired wound healing in diabetic patients.

关 键 词：高糖 保护作用 真皮 微血管内皮细胞 MCI 治疗 增殖 形态 诱导 同时检测 

分 类 号：R965[医药卫生—药理学]