自体微小颗粒骨异位移植的实验研究  

Heterotopic transplantation of autogenous minute granular bone

作　　者：马凯宇[1] 栾宏佳 徐公平[2] 张志鹏[2] 闫景龙[2] 

机构地区：[1]佳木斯市中心医院骨外科,黑龙江省佳木斯市154000 [2]哈尔滨医科大学附属第二医院脊柱关节外科,黑龙江省哈尔滨市150086

出　　处：《中国临床康复》2004年第29期6340-6342,i002,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察自体微小颗粒骨异位移植的成骨过程,以便深入研究自体颗粒骨移植的转归机制。方法:实验将40只新西兰白兔按时间随机分成0,3,10和21d4组,每组10只动物。体质量2.5～3.5kg。取自体髂骨制成直径50～100μm大小骨松质颗粒,团块样移植到同只白兔的股四头肌肌穴中。分别在颗粒骨制备的同时即(0d天组),和肌穴内移植术后,和31021d取材,进行组织学及透射电镜观察。结果:①颗粒骨制备过程中有大量细胞存留下来。②3d时一些位于微小颗粒骨边缘骨陷窝中的细胞存活。③10d时微小颗粒中骨陷窝中的骨细胞仍存活,并发生转化。④21d时形成编织骨。结论:自体微小颗粒骨直径(50～100μm)异位移植后,具有直接成骨和诱导成骨活性,能够异位成骨。AIM:To observe the osteogenesis process of heterotopic transplantation of autogenous minute granular bone, and study the mechanism of transplantation of autogenous granular bone. METHODS: Forty New Zealand rabbits,2.5 to 3.5 kg,were randomly divided into four groups: 0 , 3 , 10 and 21 day group,10 in each group.The autologous porous bone of ilium was made into granulated bone of 50 μm to 100 μm in diameter and was agglomeratively implanted into dens of quadriceps femoris and the specimen was harvested at the same time of establishment of granular bone(0 day group) and at 3,10 and 21 days after operation, and then speciment was observed by histological examination and transmission electron microscopy. RESULTS:① During the preparation of granulated bone, large quantities of osteocytes were preserved.②Three days after transplantation of dens of muscle, some osteocytes survived.③ The osteocytes still survived and transformed on the 10th day.④ The woven bone was formed on the 21st day. CONCLUSION: After the heterotopic transplantation of autogenous granular bone(50 μm to 100 μm in diameter), it has the activies of osteogenesis directly and inductive osteogenesis.

关 键 词：自体微小颗粒骨 异位移植 实验研究 异位成骨 诱导成骨 移植术 成骨过程 制备过程 活性 直接 

分 类 号：R683[医药卫生—骨科学] R658[医药卫生—外科学]