豚鼠听性脑干诱发电位对重复麻醉及不同麻醉剂的反应  

Response of auditory brainstem induced potential in guinea pigs to repeated anaesthesia and various kinds of anesthetics

作　　者：王小成[1] 冯立宁[1] 李杰[1] 任杰[1] 郭杰[1] 苟琳[1] 薛富波[2] 龙潭[1] 

机构地区：[1]解放军第四军医大学航空航天医学系临床医学教研室,陕西省西安市710033 [2]解放军第四军医大学卫生统计学教研室,陕西省西安市710033

出　　处：《中国临床康复》2004年第29期6416-6417,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:探讨短期内动物麻醉后听性脑干反应auditorybrainstemre-(sponse,ABR重复测试的可行性和理想的ABR测试麻醉剂。)方法:将11只豚鼠按随机数字法分为846合剂组(5只),戊巴比妥钠组(6只),并对动物间隔12h连续进行次麻醉,846合剂组用5846合剂腹腔注射麻醉,戊巴比妥钠组用20g/L的戊巴比妥钠溶液腹腔麻醉,并对其中846合剂组3只6只耳)和戊巴比妥钠组4只8只耳)每次((麻醉后进行ABR测试。并比较不同时间点及两种麻醉药麻醉诱导时间和维持时间以及ABR听阈和90dBSPL负短声刺激时Ⅱ波潜伏期、波幅、Ⅰ～Ⅲ波间期的变化。结果:不同时间点ABR测试各参数差异均无显著性意义;戊巴比妥钠组和846合剂组相比,90dBSPLⅡ时波潜伏期明显延长(F=5.477,P=0.037),而听阈、Ⅱ波波幅及Ⅰ～Ⅲ波间期差异无显著性意义。在不同时间点,麻醉诱导时间差异无显著性意义,而随着麻醉次数的增加,麻醉维持时间逐渐缩短(F=20.46,P=0.001);戊巴比妥钠组和846合剂组相比,麻醉起效迅速,实验后动物苏醒也快。结论:重复麻醉对豚鼠ABR测试无明显影响;846合剂是一种较为理想的豚鼠ABR测试麻醉剂。AIM:To explore the feasibility of repeated test of auditory brainstem response(ABR)in short term after anaesthesia in animals and look for ideal anesthetic for ABR test. METHODS:Eleven guinea pigs were divided into 846 mixture group(n=5) and Pentobarbital sodium group(n=6) with random number table method,and the animals were anaesthetized continuously for 5 times with an interval of 12 hours.The 846 mixture group was anaesthetized by celiac injection of 846 mixture, the Pentobarital sodium group was anaesthetized by celiac injection of Pentobarital sodium solutions 20 g/L,and 3 guinea pigs(6 ears) in the 846 mixture group and 4 guinea pigs(8 ears) in the Pentobarbital sodium group under anesthesia were tested by ABR. The changes of latency and amplitude of wave Ⅱ,and interval of wave I-Ⅲ evoked by click of 90 dB SPL and hearing threshold of ABR were compared in different groups of anesthetic and time.The time of induction and maintenance of anesthesia were also compared in the different groups. RESULTS:There was no significant difference in the parameters of ABR test at different time points.The latencies of the wave Ⅱ in 90dBSPL in the Pentobarbital sodium group were significantly prolonged compared with those of the 846 mixture group(F=5.477, P=0.037). But there was no significant difference in the hearing threshold,amplitude of wave Ⅱ and interval of wave I Ⅲ between the two groups. There was no significant difference the time of anesthesia induction at different time points. However, with the increase of frequency of anesthesia, the time of maintenance of anesthesia was shortened gradually(F=20.46, P=0.001).The effect of anesthesia acted quicker and the animals awaked faster in the Pentobarbital sodium group than in the 846 mixture group. CONCLUSION:No significant effect of repeated anesthesia is found on ABR test in guinea pigs, and 846 mixture is an ideal anesthetic for ABR test in guinea pigs.

关 键 词：麻醉剂 戊巴比妥钠 豚鼠 合剂 不同时间 听性脑干诱发电位 ABR 显著性 结论 意义 

分 类 号：R764[医药卫生—耳鼻咽喉科] R614[医药卫生—临床医学]