益气活血方对放射性骨损伤的防护作用机制(英文)  

Protective mechanism of yiqi huoxue fang against radioactive bone injury

作　　者：王炳胜[1] 张秀丽[1] 李德炳[1] 刘秀芳[1] 张海[1] 熊正文[1] 李成云[1] 

机构地区：[1]解放军第二五一医院中医肿瘤科,河北省张家口市075000

出　　处：《中国临床康复》2004年第29期6534-6535,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:临床中观察益气活血方剂有防治放射性骨损伤的作用,但其作用机制目前尚不清楚。目的:探讨益气活血方防治放射性骨损伤的作用机制。设计:随机对照的实验研究。地点和对象:在解放军第二五一医院动物试验中心及第三军医大学动物试验中心完成。48只日本大耳白兔,雌雄不限,体质量2.0～3.0kg,月龄8～12个月。干预:将48只兔随机分成实验与对照组,各24只,均60Coγ射线一次30Gy照射后肢小腿造模。诸药水煎醇沉,每毫升含生药2g。实验组兔每日胃内灌注益气活血方(组成:黄芪30g,太子参30g,白术15g,茯苓15g,丹参30g,鸡血藤30g,地龙15g,赤芍30g,甘草10g)2mL,从照射之日起,服用时间为6个月。对照组兔灌注同等量的生理盐水。主要观察指标:照射后的1,3,6,12,24,52周不同时相观察照射部位血管铸型、血流情况。结果:骨细胞变性坏死呈持续发展,第6周时尤为明显,对照组为27%,实验组为8%,差异明显。血管铸型为1～3周血管网逐增加,呈囊性扩张融合成片,以后渐减少,24周时实验组血管数目明显多于对照组。6,12,24,52周局部血流量对照组分别为(3.76±0.28),(3.02±0.22),(1.83±0.14),(1.81±0.12)L/(min·g),实验组分别为(4.46±0.35),(3.73±0.27),(3.06±0.21)(3.10±0.27)L/(min·g),与对照组比较。BACKGROUND:It is observed clinically that yiqi huoxue fang(Recipe) prevents against radioactive bone injury.But the mechanism of it is unclear yet at present. OBJECTIVE:To probe into the preventive mechanism of Recipe against radioactive bone injury. DESIGN:A randomized controlled experimental study. SETTING and PARTICIPANTS:The experiment was accomplished in Center for Laboratory Animals,the 251 Hospital of PLA and Center for Laboratory Animals,Third Military Medical Hospital.Forty eight Japanese flap eared white rabbits were employed in either gender,weighing from 2.0 to 3.0 kg,aged from 8 to 12 months. INTERVENTION:Forty eight rabbits were randomized into experimental group and control group,24 rabbits in each.All of those were radiated once only with 60Coγ rays at 30 Gy and the model was prepared on hind legs.All the herbs were decocted and sediment,each milliliter contained raw herbs 2 g.In the experimental group,gastric infusion was done daily with Recipe at 2 mL,composed of Huangqi(Radix Astragali seu Hedysari) 30 g,Taizishen(Radix Pseudostellariae) 30 g,Baizhu(Rhizoma Atractylodis Macrocephalae) 15 g,Fuling(Poria) 15 g,Danshen(Radix Salviae Miltiorrhizae) 30 g,Jixueteng(Caulis Spatholobi) 30 g,Dilong(Lumbricus) 15 g,Chishao(Radix Paeoniae Rubra) 30 g and Gancao(Radix Glycyrrhizae) 10 g.Since radiation,the Recipe was given for 6 months.In the control,the rabbits were infused with normal saline at the same dosage. MAIN OUTCOME MEASURES:To observe vascular cast and blood flow on the radiated region in the 1st,3rd,6th,12th,24th and 52nd weeks after radiation successively. RESULTS:The degenerative necrosis of osteocytes was developing,which was severer in the 6th week,27% in the control and 8% in the experimental group,indicating remarkable difference.Concerning to the vascular cast,the vascular nets were increased between the 1st and 3rd weeks,which were of vesicular dilatation and fusion into patches.Afterwards,they were decreased gradually.In the 24th week,the numbers of vessels in the experime

关 键 词：益气活血方 对照组 放射性骨损伤 照射 血流 血管铸型 骨细胞 实验 结论 等量 

分 类 号：R818.88[医药卫生—放射医学] R285.5[医药卫生—临床医学]