蝴蝶兰花梗培养的研究  

In vitro culture of Phalaenopsis flower-stalks

作　　者：伍成厚[1] 卞阿娜[1] 梁承邺[2] 叶秀[2] 

机构地区：[1]漳州师范学院生物科学与技术系,福建漳州363000 [2]中国科学院华南植物研究所,广东广州510650

出　　处：《漳州师范学院学报（自然科学版）》2004年第3期70-73,共4页Journal of ZhangZhou Teachers College（Natural Science)

基　　金：广东省重大科技专项(2003A2010401);漳州师范学院科研基金资助项目(SK03016); 福建省青年科技人才创新项目资金(2004J052)

摘　　要：以蝴蝶兰的花梗为材料,在VW、N6、MS和改良MS培养基上进行了诱导营养芽的试验.在1/4MS+BA3.0mg?L–1培养基中,蝴蝶兰花梗腋芽的萌发率为95%,培养60d后均可形成营养芽.花梗腋芽的位置和接种方式影响萌发率和营养芽的形成.The induction of vegetative shoots from axillary buds of Phalaenopsis flower-stalks was studied in vitro on the media of VW, N6, MS and some kinds of modified MS. The axillary bud sprouting rate was 95% on the medium of 1/4MS+BA3.0mg? L–1 by the culture of flower-stalk segments each with one bud. After cultured 60 days, all of the sprouting buds formed vegetative shoots. The position and the type of inoculation both make important effects on the sprouting of axillary duds and the formation of vegetative shoots.

关 键 词：蝴蝶兰 花梗 兰花 腋芽 萌发率 MS培养基 接种方式 营养 诱导 改良 

分 类 号：S682.31[农业科学—观赏园艺] S643.303.2[农业科学—园艺学]