肾上腺髓质素前体N端20肽对大鼠心律的影响  

Arrhyth miainduced by P A M Pin rat

作　　者：张人玲[1] 王容 赵志伟 姬志娟[1] 郭育红 盛树力[1] 

机构地区：[1]北京市脑老化研究实验室,北京宣武医院,100053

出　　处：《中华内分泌代谢杂志》1998年第2期109-112,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：北京市自然科学基金

摘　　要：目的　为探讨肾上腺髓质素（ Ａｄｒｅｎｏ ｍｅｄｕｌｌｉｎ ， Ａ Ｍ） 前体 Ｎ 端２２４１ 的肽（ Ｐｒｏ Ａ Ｍ２２４１ｐｅｐｔｉｄｅ， Ｐ Ａ Ｍ Ｐ） 对心律的影响。方法 　本研 究通过静 脉注入（ Ｐ Ａ Ｍ Ｐ） 和降钙 素基因相 关肽（ Ｃａｌｃｉｔｏｎｉｎ ｇｅｎｅ ｒｅｌａｔｅｄ ｐｅｐｔｉｄｅ ， Ｃ Ｇ Ｒ Ｐ） ，观察其对大鼠心律的影响。结果　 Ｐ Ａ Ｍ Ｐ 具有致心律失常的作用。在 Ｐ Ａ Ｍ Ｐ０ ．１ ～３０ ．０ｎ ｍｏｌ· Ｌ－ １·ｋｇ － １ 剂量范围内呈剂量依赖型，在３０ ．０ｎｍ ｏｌ· Ｌ－ １ ·ｋｇ － １ 时心律失常发生率为６４ ．７ ％ ，致心律失常作用与降压效果无关。小剂量的 Ｐ Ａ Ｍ Ｐ 具有加强去甲肾上腺素（ Ｎｏｒａｄｒｅｎａｌｉｎｋ ， Ｎ Ｅ） 、肾上腺素（ Ａｄｒｅｎａｌｉｎ ， Ｅ） 引起心律失常的发生率，但这种作用可被 Ｃ Ｇ Ｒ Ｐ 所拮抗。 Ｃ Ｇ Ｒ Ｐ 同样对 Ｎ Ｅ 和 Ｅ 及它们和 Ｐ Ａ Ｍ Ｐ 组合引起的心律失常的作用具有治疗效果。结论　 Ｐ Ａ Ｍ Ｐ 可能作为一种调节因子，参与心律失常的调节。Objective By intravenous administration of P A M P, the N terminal 22 41 ofadrenom edulline precursor,and calcitonin gene related peptide ( C G R P) ,their effect on heart rhyth m in ratwasinvestigated . Methods Male Wistar rats 200 250g by weight , were divided into 7 groups, underintraperitoneal anesthesia with chloral hydrate and endotracheal intubation , the left femoral artery wascannulated to record the blood pressure and the leftfe moralvein was cannulated for admimistration of normalsaline (as control) and snch drugsas P A M P, C G R P,norepinephrin ( N E)or epinephrin ( E) . The changesin E Pand E C Gfindings in terms of arrhythmia were recorded . Results P A M Pcould induce arrhyth mia in rat. The incidence of arrhyth mia at 30 . 0n mol · L- 1 ·kg - 1 P A M P was 64 . 7 % and was dose dependent in the range of 0 .1 ～30 .0nm ol· L- 1·kg - 1 . Theincidence of arrhyth mia was not related with itshypotensive effect. Sm all dose of P A M Pcould enhance obviously the incidence of arrhyth mia induced bynorepinephrin and epinephrin . But this action could be counteracted by C G R P. C G R P could decrease theincidence of arrhyth mia which wasinduced by P A M P, N Eand E, either in single of com bined use ( P< 0 .05) . Conclusion These results im ply that P A M P, as a modulating factor, takes part in the regulation ofarrhythmia .

关 键 词：PAMP 心律失常 CGRP 大鼠 肾上腺髓质素前体N端20肽 发生率 NE 拮抗 调节因子 肾上腺素 

分 类 号：R541[医药卫生—心血管疾病] R331.32[医药卫生—内科学]