新荧光试剂 CRACP 的合成及其在荧光分析中应用的研究  

Research on synthesis of new fluorescence reagent CRACP and its application in fluorometric analysis

作　　者：王忠义[1] 史海健[1] 史好新[1] 杨周生[2] 郑爱华[2] 

机构地区：[1]安徽师范大学化学系,芜湖241000 [2]安徽师范大学测试中心,芜湖241000

出　　处：《化学试剂》1993年第5期277-279,共3页Chemical Reagents

基　　金：国家自然科学基金课题

摘　　要：合成了新荧光试剂5-(4′-氯-2′-羧基苯基偶氮)罗丹宁(CRACP)。以元素分析、红外吸收光谱、核磁共振谱和质谱确定了其结构。试剂在 pH5.0～5.4时,与 Cu(Ⅱ)形成稳定的荧光螯合物,且在λ_(?)/λ_(ex)=408nm/310nm产生强荧光。其荧光强度与 Cu(Ⅱ)的浓度在0.1～0.9μg/10mL 范围内呈线性关系。检测限为0.5ng/g。已用于痕量Cu(Ⅱ)的荧光分光光度法测定。测定了茶叶中的痕量铜,结果满意。In this paper a new fluorescence reagent 5-(4'-chloro-2'-carboxylphenylazo)-rhoda- nine(CRACP) has been synthesized.Its struc- ture has been confirmed by elemental analysis, IR,NMR and MS.This reagent reacts with copper(Ⅱ) forming a stable complex in pH5.0～5.4 and shows an intense fluorescence at λ_(em)/λ_(ex)=408nm/310nm.The fluorescence intensity is proportional to copper(Ⅱ) concen tration in the range of 0.1～0.9μg/10mL.The detection limit of CRACP is 0.5 ng/g.CRACP has been applied to fluorospectrometric deter- mination of micro amounts of copper(Ⅱ) in tea with satisfactory results.

关 键 词：罗丹宁 CRACP 荧光分析 铜 

分 类 号：O657.31[理学—分析化学]