生物分子电催化的研究——Ⅱ.血红蛋白在甲酚固紫修饰石墨电极上的电催化  

Electrocatalysis of Biomolecules at Modified Electrodes Ⅱ. Catalytic Reduction of Hemoglobin at Chemically Modified Electrode Containing Cresyl Fast Violet

作　　者：韩吉林 陈洪渊[1] 高鸿[1] 

机构地区：[1]南京大学化学系,南京210008

出　　处：《化学学报》1993年第7期683-689,共7页Acta Chimica Sinica

基　　金：国家自然科学基金;南京大学现代分析中心测试基金

摘　　要：本文将甲酚固紫吸附在石墨电极上以制成修饰电极,它是一种能够加快血红蛋白在电极上电子传递速度的新的电子传递媒介体。在+0.2V～-0.4V(vs.SCE)电位范围内,吸附态的甲酚固紫表现出相当可逆的两电子氧化还原行为,同时有两个质子参加。在pH5.5 NaOAc-HOAc缓冲溶液中,其表面式量电位E^0′为-0.18V,表观电子传递速率k_s为0.71s^(-1)。甲酚固紫修饰电极具有良好的稳定性,它能够大大加快血红蛋白在电极上的电子传递速度,使其具有很好的电流响应,可应用于血红蛋白的测定。The chemically modified electrode (CME), which was constructed by adsorbing a new mediator cresyl fast violet onto the spectrographic graphite electrode, shows electrocatalytic activity to the reduction of hemoglobin. In the potential range+0.2 V～-0.4V the mediator cresyl fast violet behaves as two electrons reversible redox process involving two protons. In the medium of 0.2mol·dm^(-3)Na OAc-HOAc (pH= 5.5) the surface formal potential E°' and apparent electron-transfer rate of the adsor bed mediator cresyl fast violet are-0.18 V (vs. SCE) and 0.71s^(-1), respectively. The CME shows a good stability, and a good current response of hemoglobin. It can used as a sensor for determining hemoglobin in FIA or LC-EC method.

关 键 词：血红蛋白 修饰电极 电催化 

分 类 号：O646[理学—物理化学]