23种国产柴胡属植物中柴胡皂苷a、c、d含量的RP-HPLC测定  

Quantitative Analysis of Saikosaponin a,c,d from 23 Species of Radix Bupleuri in China by RP - HPLC

作　　者：林东昊[1] 茅仁刚[1] 王智华[2] 洪筱坤[2] 潘胜利[3] 

机构地区：[1]上海师范大学,上海新康制药厂上海200234 [2]上海中医药大学,上海200032 [3]复旦大学药学院,上海200032

出　　处：《药物分析杂志》2004年第5期479-483,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立RP-HPLC法,分离和测定中国23种61个产地的柴胡属植物样品中柴胡皂苷a、c、d的含量。方法:采用C18色谱柱(4.6 mm×250 mm,5 μm),以乙腈-水为流动相进行梯度洗脱,流速1 mL·min～1,检测波长210 nm。结果:不同品种柴胡以及相同品种、不同产地柴胡中的柴胡皂苷a、c、d含量差异悬殊。结论:本研究所建立的HPLC方法适用于柴胡属植物中柴胡皂苷a、c、d定量分析,结果准确可靠。Objective: Set up an optimal RP - HPLC method to determine the content of saikosaponin a, c, d from 23 species of 61 Radix Bupleuri samples in China. Methods;Saikosaponin a,c,d were separated by C18column(4. 6 mm ×250 mm, 5 μm) with acetonitrile -water as mobile phase in a gradient program ,flow rate was 1.0 mL· min-1 ,and detected at UV 210 nm. Results; The content of saikosaponin a,c,d of different species or the same species from various areas was significantly distinctive. Conclusion; The HPLC method is suitable for the quantitive a-nalysis of saikosaponin a.c.d.

关 键 词：柴胡皂苷A 柴胡属 含量 RP-HPLC测定 不同产地 RP-HPLC法 国产 植物 C18色谱柱 流动相 

分 类 号：R284[医药卫生—中药学] R927.2[医药卫生—中医学]