脲、盐酸胍对碳氢链疏水作用的影响  

The Effect of Guanidine Hydrochloride and Urea on the Hydrophobicity of Hydrocarbon Chain

作　　者：张兰辉[1] 李学刚[2] 

机构地区：[1]北京大学化学系,北京100871 [2]中国科学院生物物理研究所,北京100101

出　　处：《化学学报》1993年第9期869-873,共5页Acta Chimica Sinica

摘　　要：本文测定了不同脲、盐酸胍浓度下,Triton X-100溶液的临界胶团浓度(cmc)和肌酸激酶分子在溶液中的暴露巯基数.通过计算脲、盐酸胍引起的Triton X-100胶团化过程中碳氢链的疏水能改变值,得到了评价脲、盐酸胍对碳氢链疏水作用影响的参数,盐酸胍降低碳氢链疏水能的能力是脲的3.5倍.实验结果还表明,盐酸胍引起肌酸激酶内埋巯基暴露的能力约为脲的3.2倍,这意味着在一定浓度范围内脲、盐酸胍引起肌酸激酶变性的主要因素是它们降低了碳氢链的疏水作用.The critical micelle concentration (cmc) of Triton X-100 and the number of exposed thiol of creatine kinase have been measured with urea and guanidine hydrochloride (GHCl) separately as additives. The changes of hydrophobic energy of hydrocarbon chains caused by urea and GHCl in the micellization process have been calculated, and the parameter that shows the capabilities for urea and GHCl to reduce hydrophobic energy has been obtained. The capacity for GHCl to cut down the hydrophobic energy is 3.5 times as many as that for urea. The further experiment shows that the capacity for GHCl to lead to the exposure of buried thiol is 3.2 times as many as that for urea, which implies that the reduction of hydrophobic energy caused by urea and GHCl is the major factor of denaturation of creatine kinase within the certain range of additives concentrations.

关 键 词：疏水作用 盐酸胍 脲 

分 类 号：O621[理学—有机化学]