还原型谷胱甘肽对肝缺血再灌注损伤核因子-κB影响的实验研究  

Effect of glutathione on nuclear factor kappa B in rabbits with hepatic ischemia reperfusion injury

作　　者：郝云龙[1] 闻勤生[2] 魏荣荣[1] 毛兆明[1] 

机构地区：[1]武警湖北总队医院消化内科,武汉430061 [2]第四军医大学唐都医院消化科,西安710038

出　　处：《武警医学》2004年第9期661-664,共4页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨核因子-κB在肝缺血再灌注损伤中的表达和还原型谷胱甘肽的保护作用。方法将90只健康家兔随机分为对照组、肝缺血再灌注组和还原型谷胱甘肽保护组,制备肝缺血再灌注损伤模型。观察三组肝组织中核因子-κB的表达、血清ALT、丙二醛(MDA)及血浆TNF-α含量和肝脏的病理变化。结果正常肝组织中仅偶见肝细胞核因子-κB的表达,肝缺血再灌注损伤时,核因子-κB的表达明显增强,ALT、MDA、TNF-α含量显著升高(P<0.01),肝脏出现坏死,大量炎性细胞浸润;使用还原型谷胱甘肽后,上述指标的异常变化均明显减轻,其差异有显著性意义(P<0.01或P<0.05)。结论核因子-κB参与了肝缺血再灌注损伤,还原型谷胱甘肽能抑制其表达,对肝缺血再灌注损伤有积极的保护作用。Objective To investigate the expression of nuclear factor kappaB(NF κB) in hepatic ischemia-reperfusion(I/R) injury of rabbits and the protective effect of glutathione.Methods Ninety healthy rabbits were randomly divided into control group(group A),ischemia reperfusion group(group B) and glutathione protection group(group C).The rabbit model of hepatic I/R injury was developed according to Nauta. The expression of NF κB in liver tissue was determined by immunohistochemical staining,and alanine aminotransferase(ALT),methylenedioxyamphetamine(MDA),tumor necrosis factor α(TNF α) levels were assayed at 0,1,3,6 and 12 h after reperfusion,and liver tissue samples were observed with light microscope at 12 h after reperfusion.Results The expression of NF κB and the levels of ALT,MDA,and TNF α increased significantly(P<0.01),and necrosis of hepatocytes was visualized in hepatic I/R injury. There was no marked expression in control group.After treatment with glutathione, the abnormal changes of all above parameters were diminished remarkably(P<0.01 or P<0.05).Conclusion NF κB may be involved in the hepatic I/R injury.Glutathione has preventive effects against hepatic I/R injury by inhibiting the expression of NF κB.

关 键 词：肝缺血再灌注损伤 核因子-ΚB 还原型谷胱甘肽 表达 TNF-Α 保护作用 肝脏 结论 参与 显著性 

分 类 号：R657.3[医药卫生—外科学] R575[医药卫生—临床医学]