褪黑素对电离辐射诱导离体小鼠淋巴细胞凋亡的影响  

Effects of melatonin on apoptosis of mouse lymphocytes induced by ionizing radiation in vitro

作　　者：张萱[1] 龚守良[1] 王珍琦[1] 刘扬[1] 吕吉吉[1] 张铭[1] 刘树铮[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2004年第5期668-671,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题 (39970 16 5 )

摘　　要：目的 :探讨褪黑素 (m elatonin,ML T)对电离辐射诱导的离体小鼠淋巴细胞凋亡的影响及其机制。方法 :采用流式细胞术 (FCM)和荧光分光光度法分别检测小鼠胸腺和脾淋巴细胞凋亡小体百分率和 DNA裂解率。结果 :电离辐射可诱导离体小鼠胸腺、脾淋巴细胞凋亡 ,在 0 .5～ 6 .0 Gy范围内凋亡呈剂量依赖性增加。照射前预先加入 2 mm ol· L- 1 ML T,细胞凋亡小体百分率和 DNA裂解率与单纯照射组比较均显著降低 ,胸腺、脾淋巴细胞凋亡小体百分率分别降低为单纯照射组的 86 .2 5 %和 89.2 2 % ,DNA裂解率分别降低为单纯照射组的 87.2 3%和 89.16 %。结论 :ML T在体外可减轻电离辐射诱导的小鼠淋巴细胞凋亡 ,对淋巴细胞损伤有防护作用。Objective To explore the effects of melatonin (MLT) on the apoptosis of mouse lymphocytes in vitro induced by ionizing radiation and its mechanism. Methods The percentages of apoptotic bodies and the DNA lytic rates in mouse thymocytes and splenocytes were detected with flow cytometry and fluorospectrophotometry, respectively. Results The apoptosis of mouse thymocytes and splenocytes increased in a dose-dependent manner in 0.5~6.0 Gy X-irradiation. When MLT of 2 mmol·L -1 was added into thymocytes or splenocytes in vitro before irradiation with 0.5~6.0 Gy X-rays, the percentages of apoptotic bodies and the DNA lytic rates all decreased significantly compared with those in the irradiation group. The percentages of apoptotic bodies in these two kinds of cells were 86.25% and 89.22% of those in the irradiation group, respectively, and the DNA lytic rates were 87.23% and 89.16%, respectively. Conclusion MLT can decrease the apoptosis of mouse lymphocytes and has the protective effects on the damage of lymphocytes.

关 键 词：褪黑激素 生理学 辐射 电离 细胞凋亡/药物作用 淋巴细胞/药物作用 小鼠 

分 类 号：Q691[生物学—生物物理学]