子宫内膜异位症模型大鼠异位组织中巨噬细胞和凋亡细胞数的变化及活血化瘀中药的影响  

Changes of macrophages and apoptosis in rat endometriosis model and effects of traditional Chinese medicine

作　　者：徐邦生[1] 蔡云平[2] 朱建华[3] 李奕[2] 

机构地区：[1]南通医学院分子神经生物学江苏省重点实验室,南通226001 [2]南通医学院组织胚胎学教研室 [3]南通医学院附属医院中医科

出　　处：《解剖学杂志》2004年第5期472-475,F002,共5页Chinese Journal of Anatomy

基　　金：江苏省普通高校自然科学研究计划 (0 2KJD310 0 0 1)

摘　　要：目的 :探讨巨噬细胞与子宫内膜异位症 (EMT)的关系及活血化瘀中药对其的影响。方法 :制作大鼠EMT动物模型 ,模型鼠连续用药 3w后观察在位、异位组织中巨噬细胞和凋亡细胞数的变化以及异位子宫内膜上皮及腺上皮细胞的凋亡指数。结果 :模型对照组内膜巨噬细胞数比正常大鼠增多 ,异位比在位组织中增多非常显著。中药治疗前后巨噬细胞数无显著变化。模型对照组细胞凋亡指数 ,基质细胞凋亡数非常显著小于用药组。结论 :活血化瘀中药能促进异位组织消退 ,促进异位内膜细胞凋亡。Objective: To study the changes of macrophages in endometriosis (EMT) model rats with and without traditional Chinese medicine and the relationship between apoptosis and macrophages. Methods: EMT rat model was established. EMT rats were given traditional Chinese medicine for 3 weeks. The changes of macrophages in the ectopic and eutopic endometrium and the relationship between apoptosis and the number of macrophages were all observed. Results: The number of macrophages in normal rat was significantly lower than that in the EMT model rats and experimental groups. The number of macrophages in the eutopic was lower than in the ectopic. There were no distinct changes between the groups with and without traditional Chinese medicine. The apoptotic indices were (20.08±0.38)% in 0.5 ml experimental group and (20.74±0.52)% in 1.0 ml group. Few apoptotic cells were found in control group. Conclusion: Traditional Chinese medicine promotes apoptosis in the ectopic tissue. The increase of apoptotic index in endometrium of EMT model rats may be due to the increase of macrophage activity.

关 键 词：巨噬细胞 活血化瘀中药 子宫内膜异位症 凋亡细胞 EMT 对照组 模型大鼠 结论 变化 促进 

分 类 号：R711.71[医药卫生—妇产科学] R587.1[医药卫生—临床医学]