大鼠杏仁体基底外侧核GABA和ACh能中间神经元的突触  

Synapses on GABAergic and cholinergic interneurons in basolateral nucleus of rat amygdala

作　　者：李瑞锡[1] 彭裕文[1] 大谷修 西条寿夫 王劼[1] 丁忠良[1] 高璐[1] 

机构地区：[1]复旦大学上海医学院解剖学与组织胚胎学系,上海200032 [2]日本国富山医科药科大学解剖学 [3]日本国富山医科药科大学生理学讲座

出　　处：《解剖学杂志》2004年第5期509-513,527,共6页Chinese Journal of Anatomy

基　　金：复旦大学引进人才基金 (EXF 10 2 30 2 )

摘　　要：目的 :揭示杏仁体基底外侧核 (BL)中的γ 氨基丁酸 (GABA)能和乙酰胆碱能 (ACh) 2种中间神经元树突上的突触联系。方法 :用抗GABA和抗胆碱乙酰转移酶 (ChAT)抗体对BL做光镜和电镜免疫组化染色。结果 :光镜下 ,GABA免疫反应阳性神经元多为圆形多极神经元 ;ChAT免疫阳性神经元多为双极神经元。两者数量比约为 ( 7～ 9)∶1。电镜下 ,支配GABA免疫阳性神经元的突触 5 2 .7%为非对称性 (兴奋性 ) ,47.3 %为对称性 (抑制性 ) ,而ChAT免疫阳性神经元则分别为 44.9%和 5 5 .1 %。结论 :在情绪性学习记忆的处理过程中 ,杏仁体GABA能中间神经元起主要的功能作用 ,可能为抑制作用 ;Objective: To reveal the synaptic connections on the γ-aminobutyric acid (GABA)ergic and acetyl cholinergic (ACh) interneurons in the basolateral nucleus (BL) of the amygdala. Methods: Immunohistochemical methods were employed to label the GABAergic and ACh neurons in the BL with anti-GABA and anti-choline acetyltransferase (ChAT) antibodies for both light and electron microscopy. Results: At the LM level, majority of the GABA-immunoreactive (IR) interneurons revealed as round multipolar neurons, while most of the ChAT-IR interneurons were bipolar. The numbers of GABA-IR versus ChAT-IR interneurons were approximately 7-9 to 1 in a given section of 35μm thickness. Under EM, GABA-IR interneurons were innervated with 52.7% asymmetric (excitatory) and 47.3% symmetric (inhibitory) synapses. Comparatively, the ChAT-IR interneurons received about 44.9% asymmetric and 55.1% symmetric synaptic inputs. Conclusion: The GABAergic neurons constitute a dominant population of the local circuit interneurons with the ACh interneurons as subsidiary elements in the BL of amygdala. The GABAergic neurons exert an inhibitory, while the ACh interneurons exert a neutral (balance in excitatory and inhibitory) influence to the amygdalar neurons during emotional learning and memory process.

关 键 词：中间神经元 γ-氨基丁酸(GABA) 杏仁体 免疫阳性神经元 突触联系 电镜 光镜 外侧核 树突 BL 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]