大鼠自体移植脾组织GAP-43^+神经再生的实验研究  

Experimental study on regeneration of GAP-43 positive nerve fibers in splenic autotransplantation

作　　者：郭光金[1] 王林[1] 蒋登金[1] 左艳芳[1] 张天飞[1] 

机构地区：[1]第三军医大学基础医学部外科应用解剖与手术学教研室,重庆400038

出　　处：《局解手术学杂志》2004年第5期289-291,共3页Journal of Regional Anatomy and Operative Surgery

基　　金：重庆市科委攻关课题 ( 2 0 0 3 )

摘　　要：目的　研究大鼠自体脾组织移植术后移植脾组织GAP 43 + 神经纤维再生及分布规律。方法　健康Wistar大鼠 10 5只 ,随机分为自体脾组织移植组和对照组 ,于术后 7、15、3 0、60、90、12 0、180d取移植脾组织标本 ,应用免疫组化方法显示GAP 43 + 神经纤维 ,并用图像分析系统 ,对不同时相点免疫组化GAP 43 + 染色区域进行图像定量分析。结果　自体脾组织移植术后 3 0d ,移植脾组织周围大网膜内可见GAP 43 + 神经纤维 ,并向移植脾组织内伸展 ;自体脾组织移植术后 60～ 12 0d ,移植脾组织内再生神经纤维逐渐增多 ;术后 180d ,移植脾组织中GAP 43 + 神经纤维分布及密度接近正常。结论　GAP 43 + 神经纤维于脾移植术后 60d开始出现 ,术后 180dGAP 43 +Objective To explore the regeneration of growth associated protein 43 (GAP-43) positive nerve fibers in splenic autotransplantation in rats. Methods Healthy Wistar rats were randomly divided into two groups: splenectomy plus splenic autotransplantation group, and sham-operation group. The regeneration of GAP-43 positive nerve fibers was observed by immunohistochemical method at 7, 14, 30, 60, 90, 120, and 180 d after operation. Computer image analysis was conducted for analysis of the density changes. Results The GAP-43 nerve fibers appeared in the greater omentum at 30 d after operation, and the density of nerve fibers became greater at 60-120 d and became almost normal on 180 d after operation. Conclusion GAP-43 nerve fibers are found in the autotransplant tissues at 60 d and become almost normal at 180 d after splenic autotransplantation.

关 键 词：自体脾组织移植 GAP-43 术后 神经纤维 大鼠 正常 自体移植 结论 时相 目的 

分 类 号：R657[医药卫生—外科学] R651[医药卫生—临床医学]