电针足三里对大鼠脑肠肽含量的影响及神经免疫调控作用(英文)  

Effect of electro-acupuncture at Tsusanli on the content of braingut peptide in rats and its regulatory function of neuroimmunology

作　　者：高巍[1] 黄裕新[1] 陈洪[2] 王庆莉[1] 

机构地区：[1]解放军第四军医大学唐都医院消化内科,陕西省西安市710038 [2]解放军第四军医大学唐都医院医教部,陕西省西安市710038

出　　处：《中国临床康复》2004年第31期7072-7074,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助项目(39570888)~~

摘　　要：背景:针刺对红细胞免疫和T细胞亚群均有正向调节作用,但其机制尚未完全阐明。目的:探讨免疫系统在针刺治疗相关疾病中的调控作用及可能的机制。设计:随机对照实验研究。地点和对象:实验地点:解放军第四军医大学唐都医院消化内科实验室。将SD大鼠40只随机分成正常对照组、非经非穴组、免疫抑制组、足三里组、足三里+免疫抑制组(n=8)。干预:应用放射免疫法测定脑垂体和外周血P物质放免活性(ir-SP)和血管活性肠肽放免活性(ir-VIP)含量的变化;应用流式细胞仪技术,通过微量全血直接免疫荧光染色法测定外周血T细胞亚群以反映细胞免疫功能;应用红细胞C3b受体-酵母菌花环试验和红细胞-IC花环试验检测红细胞免疫功能。主要观察指标:各组大鼠的ir-SP和ir-VIP含量;T细胞亚群和红细胞免疫功能的变化。结果:足三里组大鼠外周血和脑垂体中P物质(substanceP,SP)、血管活性肠肽(vasoactiveintestinalpeptide,VIP)含量犤外周血SP:(591.83±142.59)pg/L,VIP(92.05±22.11)pg/L;脑垂体SP:(47.00±9.12)pg/L,VIP(308.36±34.16)pg/L、T4(CD4+)细胞百分率犤(65.55±8.42)%犦、C3b受体花环率(RBC-C3bRR)犤(15.88±2.99)%犦、红细胞免疫复合物花环率(RBC-ICR)犤(12.14±1.22)%犦明显高于正常对照组犤(569.36±8.04)pg/L,(20.45?BACKGROUND: Acupuncture can positively regulate both the erythrocytic immunity and T lymphocyte subgroups.But its mechanism still remains to be interpreted OBJECTIVE: To investigate the regulatory role of immune system in the course of electro-acupuncture(EA) in treating relative diseases as well as its mechanism. DESIGN: Randomized and controlled experimental study. SETTING and MATERIALS:Experimental setting:Laboratory of Digestive Diseases,Tangdu Hospital, Fourth Military Medical University of Chinese PLA.Forty rats were randomly divided as normal control group, non-acupoint group,immunosuppressive group,Tsusanli group,and Tsusanli+immunosuppressed group,with 8 rats in each group. INTERVENTIONS:The activity of immunologic response of substance P (ir-SP) and that of vasoactive intestinal peptide (ir-VIP) in rats' pituitary gland and peripheral blood were detected with radioimmunoassay;The changes of T-lymphocytic subgroups were assayed with micro-whole-blood direct immunofluorescence staining and flow cytometry technique.Erythrocytic immunity function was assayed by red blood cell C3b receptor rosette(RBC-C3bRR) and red blood cell immune complexes rosette (RBC-ICR). MAIN OUTCOME MEASURES:Contents of ir-SP and ir-VIP and changes of T-lymphocytic subgroups and the immune function of erythrocyte. RESULTS: The contents of SP and VIP in the peripheral blood and hypophysis [(591.83±142.59) pg/L,(92.05±22.11) pg/L,respectively,in the peripheral blood;(47.00±9.12) pg/L,(308.36±34.16) pg/L,respectively,in the hypophysis], the percentage of T4(CD4+)[(65.55±8.42)%],the rosette rate of red blood cell C3b receptor(RBC-C3bRR)[(15.88±2.99)%],and red blood cell immune complexes rosette (RBC-ICR)[(12.14±1.22)%] in the Tsusanli group were significantly higher than those in the normal control group [(569.36±8.04) pg/L, (20.45±6.74) pg/L,(27.86±7.67) pg/L,(236.71±22.83) pg/L, (43.06±3.14)%,(8.63±2.13)%,(6.75±2.32)%,respectively](P< 0.01).And the CD4+was positively correlated with the changes of the contents

关 键 词：足三里 外周血 VIP CD4+ 对照组 正常 脑垂体 百分率 结论 RBC-C3BRR 

分 类 号：R392.1[医药卫生—免疫学] R512[医药卫生—基础医学]