Tp-19(s)-IgM-ELISA诊断早期先天梅毒  

Treponema pallidum 19(s)-IgM-enzyme-linked immunosorbent assay laboratory diagnosis of early congenital syphilis

作　　者：何君[1] 徐明茹[1] 

机构地区：[1]南京市疾病预防控制中心皮肤病性病专科医院检验科,江苏南京210015

出　　处：《现代医学》2004年第5期319-321,共3页Modern Medical Journal

摘　　要：目的　通过梅毒几种检测方法的比较 ,寻找一种诊断早期先天梅毒的方法。方法　用梅毒甲苯胺红不加热血清实验 (TRUST)、梅毒螺旋体颗粒凝集实验 (TPPA)和梅毒螺旋体 19(s) IgM酶联免疫吸附法 [Tp 19(s) IgM ELISA]对 6例血清快速血浆反应素环状卡片试验 (RPR)阳性新生儿 (其母亲已在怀孕时被确诊为不同病期梅毒 )的血清进行检测。结果　 6例新生儿中有 4例TRUST、TPPA、Tp 19(s) IgM阳性 ;另 2例TRUST、TPPA均阳性 ,但Tp 19(s) IgM阴性。前 4例新生儿确诊为早期先天梅毒 ,后面 2例排除早期先天梅毒。结论　特异性IgM检测应该作为早期先天梅毒确诊的实验诊断方法。Objective To search for a diagnostic method for early congenital syphilis by comparing of several detecting methods. Methods Toludine red unheated serum test(TRUST),Treponema pallidum particle agglutination (TPPA) and Treponema pallidum 19(s)?IgM enzyme-linked immunosorbent assay [Tp?19(s)?IgM?ELISA] were used to detect the serums of 6 newborns whose serums were rapid plasma reagin(RPR) positive and whose mothers were diagnosed with syphilis at different stages. Results Four of them were TRUST,TPPA and Tp?19(s)?IgM?ELISA positive and finally they were diagnosed with early congenital syphilis, the other 2 were TRUST,TPPA positive but Tp?19(s)?IgM?ELISA negative and were excluded early congenital syphilis. Conclusion It is suggested that specific IgM test should be used as confirmatory laboratory diagnostic method for early asymptomatic congenital syphilis.

关 键 词：早期先天梅毒 诊断 TRUST ELISA TPPA 确诊 阳性 结论 方法 实验 

分 类 号：R759[医药卫生—皮肤病学与性病学] R446[医药卫生—临床医学]