空气细颗粒物与慢性鼻窦炎组织形态学相关关系的初探  

Preliminary study on the relationship between chronic sinusitis histomorphology and aerosol

作　　者：刘红刚[1] 何春燕[1] 徐东群[2] 王先忠[1] 梁乐[1] 陈红[1] 

机构地区：[1]首都医科大学附属北京同仁医院病理科,北京100730 [2]中国疾病预防控制中心环境与健康相关产品安全所空气质量安全监测室

出　　处：《中华劳动卫生职业病杂志》2004年第5期340-342,F005,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目(20077033);北京市自然科学基金资助项目(7992018);2003年北京市留学回国人员科技活动项目择优资助经费资助项目

摘　　要：目的 探讨空气颗粒物与慢性鼻窦炎(CS)组织形态学改变的关系。方法 收集CS Ⅰ、Ⅱ、Ⅲ型筛窦病变黏膜组织共60例、正常筛窦黏膜对照组织16例、成人及胎儿尸检肺组织各5例。对上述标本分别行HE染色及Warthin-Starry(W-S)空气颗粒物特殊染色,另对CSⅠ、Ⅱ、Ⅲ型病变黏膜行VG染色显示并测量基底膜的厚度。结果W-S染色发现,在各型CS病变黏膜上皮表层及损伤脱落处细胞内均有黑色空气颗粒物沉积。颗粒沉积的程度在CSⅠ、Ⅱ、Ⅲ型间逐渐加重,轻、中、重度沉积的例数:Ⅰ型分别为11/20、7/20、2/20例;Ⅱ型分别为5/20、9/20、6/20例;Ⅲ型分别为3/20、6/20、11/20例。颗粒沉积程度越重,其基底膜越厚,Ⅰ、Ⅱ、Ⅲ型基底膜厚度分别为(12.15±4.73)、(16.67±2.22)、(23.75±3.48)μm,3型之间的差异有统计学意义(P<0.01)。没有损伤的黏膜上皮细胞及对照组正常筛窦黏膜上皮层内极少见到此空气颗粒物。结论 在CS病变黏膜上皮表层及细胞内存在空气颗粒物,此颗粒物的存在与CS病变的发生可能有关。Objective To observe the relationship between histomorphological changes in mucosa of chronic sinusitis(CS) and aerosol particles. Methods The ethmoidal sinus mucus specimens of 60 patients with CS type Ⅰ , Ⅱ and Ⅲ , and 16 cases of normal controls were collected. Meanwhile the pulmonary specimens in autopsy of 5 adult and 5 fetus cases were also collected. The specimens were stained by HE staining and Warthin-Star-ry(W-S) special aerosol particles staining method respectively, the ethmoidal sinus mucosa basement membrane were displayed by Van Gieson's(VG) special staining method,and its thickness was measured. Results By the W-S staining, some black aerosol particles were found depositing on the epithelial cells layer of damaged mucosa and in the plasm of the epithelial cells. The degree of deposition was gradually increased in type Ⅰ, Ⅱ and Ⅲ of CS. The number of cases of slight,medium and heavy depositing in type Ⅰ were 11/20,7/20,2/20,in type Ⅱ 5/20,9/20, 6/20,and in type Ⅲ 3/20,6/20,11/20 respectively; the more particles deposited, the more thickening the basement membrane. The thickness of the basement membrane in type Ⅰ , Ⅱ and Ⅲ of CS were (12.15 ± 4. 73), (16.67 ± 2.22) , (23 .75 ± 3 .48)μm respectively. There were significant differences among type Ⅰ , Ⅱ and Ⅲ (P < 0.01) .The black particles in the mucosa epithelium tissues of normal control group and non-damaged mucosa epithelium and desquamation area were seldom seen. On the other hand, the aerosol particles were not seen in the pulmonary specimens of the fetal autopsy. Conclusion In damaged mucosa epithelial cells of the CS, there are aerosol particles existing which may relate with the morphological changes of CS.

关 键 词：黏膜 筛窦 组织形态学 基底膜 慢性鼻窦炎 对照组 正常 空气颗粒物 细颗粒物 沉积 

分 类 号：R765.4[医药卫生—耳鼻咽喉科]