云南省不同地理株恶性疟原虫的MSP-2基因多态性研究  

Study on MSP-2 gene polymorphisms of different geographical strains of Plasmodium faciparum in Yunnan

作　　者：杨亚明[1] 张国森[1] 刘慧[1] 张再兴[1] 高白荷[1] 

机构地区：[1]云南省寄生虫病防治所,云南思茅665000

出　　处：《中国热带医学》2004年第6期911-912,906,共3页China Tropical Medicine

摘　　要：目的　研究云南省不同地理株恶性疟原虫的裂殖子表面抗原蛋白基因 2 (MSP -2 )的多态性 ,探索其多态性的地域特征 ,为鉴定不同地理株恶性疟原虫提供重要的科学依据。　方法　使用随机扩增多态DNA -多聚酶链反应(RAPD -PCR)为主的分子生物学方法。　结果　分析云南不同疟疾流行区的 169份样本 ,PCR扩增获得 6个不同大小的MSP -2基因片段 ,60 0bp片段占多 ,其频度为 3 4 8% ,5 40bp片段其次 ,占 2 8 3 % ;同时显示具有一定的地理分布差异。　结论　研究证实云南不同地区恶性疟原虫的MSP -2具有较为明显的地理多态性特征。Aim To study on MSP-2 gene polymorphisms of different geographical strains of Plasmodium falciparum in Yunnan and explore geographical characteristics of their polymorphisms so that to provide an important scientific evidence for identifying different geographical strains of Plasmodium falciparum. Methods Random Amplified DNA Polymorphism-PCR techniques were used. Results 169 samples collectied from different areas in Yunnan, were analyzed in Yunnan and 6 MSP-2 fragments of different sizes were obtained after amplified by PCR. 600bp fragments is mainly types, with a frequency of 34.8%. 540bp fragments were the secondary with a frequency of 28.3%. These polymorphisms also showed certain differences of distribution in Yunnan. Conclusion The study indicated that MSP-2 gene polymorphisms of different geographical strains of Plasmodium falciparum in Yunnan is evident.

关 键 词：恶性疟原虫 MSP 地理株 基因多态性 疟疾 表面抗原 流行区 随机扩增多态DNA 蛋白基因 RAPD-PCR 

分 类 号：R382.31[医药卫生—医学寄生虫学] R384.1[医药卫生—基础医学]