HPLC法测定联磺甲氧苄啶片中三种组分的含量  

Determination of Three Components in Sulfamethoxazole Sulfadiazine and Trimethoprim Tablets by HPLC

作　　者：林丽红 徐灿辉 

机构地区：[1]湖南省益阳市药品检验所,益阳413000

出　　处：《中国药品标准》2004年第5期37-39,共3页Drug Standards of China

摘　　要：目的:建立了高效液相色谱法测定联磺甲氧苄啶片中磺胺甲(口恶)唑、磺胺嘧啶与甲氧苄啶的含量。方法:采用Kro-masilTMC18柱,以水-乙腈-三乙胺(800:200:1),用0.2mol·L-1氢氧化钠和稀醋酸调pH值至6.6±0.1为流动相,流速1ml·min-1,检测波长为210nm,结果:磺胺甲(口恶)唑、磺胺嘧啶的线性范围均为10.0-100.0μg·ml-1,甲氧苄啶的线性范围4.0-40.0μg·ml-1,平均回收率分别为99.9%(RSD=0.68%)、99.6%(RSD=0.77%)和99.7%(0.74%)。结论:本方法快速、简便、准确,可作为该制剂质控标准。Objective:To establish HPLC method for the determination of SD and SMZ and TMP in sulfamethoxazole,sulfadi-azine and trimethoprim tablets. Method:Using KromasilTM C18 Columns. Water-acetonitrile-triethylamine(800 :200 : 1) was adjusted to pH 6. 6±0. 1 with 0. 2mol · L-1 sodium hydroxide and acetic acid as mobile phase,at a flow rate of 1.0ml· min-1, wavelength of the detector was 210nm. Results: Linear ranges of SMZ and SD and TMP were 10. 0-100. 0μg· ml-1,10. 0-100.0μg · ml-1,4.0-40.0μg · ml-1, respectively. The average recoveries of SMZ and SD and TMP were 99.9%(RSD = 0.68%),99.6%(RSD = 0. 77%) and 99. 7% (0.74%),respectively. Conclusion: The method is rapid,simple and accurate. The method is suitable for the quality control of this preparation.

关 键 词：甲氧苄啶 磺胺甲噁唑 HPLC法 含量 RSD 磺胺嘧啶 质控标准 组分 三乙胺 氢氧化钠 

分 类 号：R927[医药卫生—药学] R286.0