HPLC法测定人胎盘片原料中腺苷含量  

Determination of Adenosine in Human Placenta by HPLC

作　　者：纪宏[1] 高春[1] 高恒莹[1] 

机构地区：[1]北京市药检所,北京100035

出　　处：《中国药品标准》2004年第5期49-51,共3页Drug Standards of China

摘　　要：目的:建立人胎盘(紫河车)中腺苷的反相高效液相色谱检测法。方法:十八烷基硅烷键合硅胶色谱柱;以0.02mol／L磷酸二氢钾溶液(A液),0.06mol／L磷酸二氢钾溶液-甲醇-四氢呋喃(89:10:1)(B液)为流动相,梯度洗脱;检测波长:260nm。结果:线性范围:0.1-1.0μg,r=0.9999,回收率为99.3%,RSD为1.02%(n=9)。结论:该方法结果准确,重现性好,可用于人胎盘片原料(紫河车)的质量控制。Objective: A HPLC method was described for the content determination of adenosine in human placenta. Method: C18 column; with potassium dihydrophosphate solution (0. 02mol/L) (Solution A), potasssium dihydrophosphate solution (0.06mol/L)-methanol-tetrahydrofuran(89:10 :1)(Solution B)as mobile phase,gradient separation; UV detector wavelength :260nm. Results: The standard curve was liner in the range of 0. 1-1.0μg of adenosine(r = 0.9999). The average recovery of adenosine was 99. 3% ,RSD=1.02%(n = 9). Conclusions:The established method is rapid,accurate and stable. This has important meaning for the internal quality control in raw materials and preparations of human placenta.

关 键 词：紫河车 HPLC法 腺苷 含量 梯度洗脱 RSD 反相高效液相色谱 原料 硅烷 四氢呋喃 

分 类 号：R284[医药卫生—中药学] R286.0[医药卫生—中医学]