有氧运动对胰岛素抵抗大鼠脂肪组织蛋白激酶B表达的影响  

Effect of exercise on expression of protein kinase B in adipose tissue of rats

作　　者：李建辉[1] 毕会民[1] 甘佩珍[1] 汪保华[2] 

机构地区：[1]武汉大学人民医院内分泌科,湖北武汉市430060 [2]荆门市第一人民医院神经内科,湖北荆门市448000

出　　处：《中国康复理论与实践》2004年第10期585-587,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：湖北省自然科学基金项目 (No.2 0 0 3ABA1 82 )

摘　　要：目的在高脂饮食诱导胰岛素抵抗的基础上 ,观察有氧运动对胰岛素抵抗大鼠脂肪组织中蛋白激酶B(PKB)表达的影响。方法选取雄性Wistar大鼠 3 0只 ,随机分为正常对照组 10只 ,给予基础饲料 ;模型组 2 0只 ,给予高脂饲料喂养 4周后 ,再随机分为胰岛素抵抗组 (继续高脂饮食 )和运动处理组 (高脂饮食 +游泳运动 )。干预 6周后 ,采用蛋白印迹法检测大鼠脂肪组织中胰岛素刺激PKB表达的含量。结果在胰岛素抵抗状态时 ,大鼠脂肪组织PKB表达明显减少 ,较对照组下降 2 3 .5 % (P <0 .0 1) ;6周游泳运动后 ,运动组大鼠PKB表达明显增加 ,较胰岛素抵抗组增加 19.2 % (P <0 .0 1)。结论运动能改善胰岛素抵抗 。ObjectiveTo observe the effect of exercise on expression of protein kinase B (PKB) in adipose tissue of insulin resistant rats fed by high fat diet.Methods30 male Wistar rats were randomly divided into the control group (n=10),given basic diet; the model group (n=20), given fat diet. After 4 weeks, the model group was randomly divided into 2 subgroups, the insulin resistant group was continually given high fat diet, the exercise treated group accepted high fat food and swimming training. After 6 week intervention, the expression of PKB stimulated by insulin in adipose tissue was determined with Western blotting at the end of experiment.ResultsAfter long term high fat diet, expression of PKB in adipose tissue of the insulin resistant group decreased by 23.5% comparing with the control group ( P<0.01). After 6 weeks swimming training, the expression of PKB of the exercise treated group was increased by 19.2% comparing with the insulin resistant group ( P<0.01).ConclusionExercise treatment can significantly elevate the expression of PKB and ameliorate the state of insulin resistance.

关 键 词：运动 胰岛素抵抗 脂肪组织 蛋白激酶B 

分 类 号：R458[医药卫生—治疗学]