HPLC同时测定肉苁蓉药材中松果菊苷和毛蕊花糖苷的含量  

A RP-HPLC method for simultaneous determination of echinacoside and acteoside in Herbe Cistanches

作　　者：张思巨[1] 刘丽[1] 于江泳[1] 崔光红[1] 

机构地区：[1]中国中医研究院中药研究所,北京100700

出　　处：《中国药学杂志》2004年第10期740-741,共2页Chinese Pharmaceutical Journal

基　　金：中央级科研院所科技基础性工作专项

摘　　要：目的建立RP-HPLC同时测定肉苁蓉中松果菊苷和毛蕊花糖苷的含量.方法采用HP-1100色谱系统和YMC-PackODS-A柱(5μm,25mm×4.6 mm);以CH3CN-MeOH-1%HAc(10:15:75)为流动相,流速0.7mL·min-1,检测波长为334nm,柱温:30℃.结果松果菊苷进样量在0.12～1.47μg内、毛蕊花糖苷在0.18～2.16μg内与峰面积呈良好线性关系,相关系数均为0.9999;平均回收率分别为98.87%和97.01%.结论本方法简便,精密度、重现性良好,结果准确可靠.可用于肉苁蓉药材、饮片及其制剂的含量测定.OBJECTIVE: To establish a RP-HPLC method for the simultaneous determination of echinacoside and acteoside in Herbe Cistanches. METHODS: HP - 1100 HPLC system and YMC - Pack ODS-A column(5 μm, 4.6 mm × 250 mm) were adopted. The mobile phase consisted of CH3CN-MeOH-1% HAc(10:15:75) and the detection wavelength was 334 nm with a PDA detector. The flow rate was 0.7 mL&middot min-1 and the column temperature was 30°C. RESULTS: The linear ranges of echinacoside and acteoside were 0.12-1.47 μg (r = 0.999 9) and 0.18-2.16 μg (r = 0.999 9), respectively. The average recoveries(n = 5) of echinacoside and acteoside were 98.87% (RSD% = 1.85) and 97.01% (RSD% = 0.97) respectively. CONCLUSION: This method is simple, accurate and reliable. It can be used as a quantitative determination method for the quality control of Herbe Cistanches and its preparation.

关 键 词：肉苁蓉 松果菊苷 毛蕊花糖苷 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]