Study on the Reaction of Proteins with 5-Nitrosalicylfluorone-molybdenum(Ⅵ) Complex by Spectrophotometry in PVA 124 Microemulsion  

作　　者：魏琴 吴丹 杜斌 张慧 欧庆瑜Lanzhou Institute of Chemical Physics Chinese Academy of Sciences Lanzhou Gansu 730000 China 

机构地区：[1]School of Chemistry & Chemical Engineering, Jinan University, Jinan, Shandong 250022, China Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China [2]School of Chemistry & Chemical Engineering, Jinan University, Jinan, Shandong 250022, China

出　　处：《Chinese Journal of Chemistry》2004年第7期714-718,615,共5页中国化学（英文版）

基　　金：Project supported by the Natural Science Foundation of Shandong Province (No. Y2000B09) and the Science and Technology Programme of Shandong Province (No. 03C05).

摘　　要：The application of 5'-nitrosalicylfluorone (5'-NSF)-molybdenum(VI) complex as a spectroscopic probe was studied. In the buffer medium of HOAc-NaOAc at 3.45 and in the presence of PVA 124 microemulsion, 5'-NSF-Mo(VI) complex combines protein rapidly to form a stable compound, leading to an absorbance decrease at 525 nm of 5'-NSF-Mo(VI) complex. According to this change, microdetermination of protein has been described. Bovine serum albumin (BSA) could be determined in the linear range of 0—16 g昺L-1 with the detection limit of 11 ngmL-1. Many amino acid and metal ions studied do not interfere with the assay. The method possesses high sensitivity as well as high selectivity. It can be used to determine protein in human urine and milk powder successfully. The relative standard deviations are in all instances less than 4.7%, and the recoveries are between 97.6% and 106.0%. Moreover, the binding number of BSA with the complex, which is determined by using molar ratio, Rosenthanl graphic and slope ratio methods, is in good agreement with each other.The application of 5'-nitrosalicylfluorone (5'-NSF)-molybdenum(VI) complex as a spectroscopic probe was studied. In the buffer medium of HOAc-NaOAc at 3.45 and in the presence of PVA 124 microemulsion, 5'-NSF-Mo(VI) complex combines protein rapidly to form a stable compound, leading to an absorbance decrease at 525 nm of 5'-NSF-Mo(VI) complex. According to this change, microdetermination of protein has been described. Bovine serum albumin (BSA) could be determined in the linear range of 0—16 g昺L-1 with the detection limit of 11 ngmL-1. Many amino acid and metal ions studied do not interfere with the assay. The method possesses high sensitivity as well as high selectivity. It can be used to determine protein in human urine and milk powder successfully. The relative standard deviations are in all instances less than 4.7%, and the recoveries are between 97.6% and 106.0%. Moreover, the binding number of BSA with the complex, which is determined by using molar ratio, Rosenthanl graphic and slope ratio methods, is in good agreement with each other.

关 键 词：protein SPECTROPHOTOMETRY 5'-NSF-Mo(VI) complex MICROEMULSION 

分 类 号：O657.3[理学—分析化学]