MTT法检测通心络对血管内皮细胞增殖的影响  

The Effect of Tong-Xin Luo on Human Umbilical Vein Endothelial Cells Proliferation

作　　者：杨敏[1] 孙明[1] 周宏研[1] 刘遂心[1] 何飞[1] 李彤[1] 

机构地区：[1]中南大学湘雅医院心内科,湖南长沙410008

出　　处：《中国医师杂志》2004年第10期1329-1330,共2页Journal of Chinese Physician

摘　　要：目的　研究通心络 (TXL)对体外培养的人脐静脉内皮细胞 (ECV -3 0 4)增殖的影响。方法　制备通心络含药血清 ,用5 % ,10 % ,15 %TXL含药血清分别作用培养的ECV -3 0 4细胞 4h ,8h。通过细胞形态学、MTT法检测TXL含药血清对ECV -3 0 4细胞增殖的影响。结果　通心络组光密度 (OD)值明显高于对照组 (P <0 0 1) ,含药血清培养 8h的OD值明显高于 4h的 (P <0 0 5 )。结论　TXL促进ECV -3 0 4细胞增殖。Objective To investigate the effects of Tong-Xin Luo (TXL) on the proliferation of human umbilical vein endothelial cells (ECV-304) cultured in vitro. Methods The TXL-contained serum was prepared, and ECV-304 were incubated with 5%, 10% and 15% serum containing TXL, respectively, for 4 hours and 8 hours. The cell morphology was observed and cell proliferation ability was determined by MTT. Results OD value of TXL group was higher than that in control group(P<0.01). OD value of the cells cultured in TXL-contained serum for 8 hours was higher than that cultured for 4 hours(P<0.05 ). Conclusion Tong-Xing Luo can promote human umbilical vein endothelial cells proliferation.

关 键 词：通心络 含药血清 增殖 MTT法 血管内皮细胞 ECV-304细胞 影响 体外培养 人脐静脉内皮细胞 细胞形态学 

分 类 号：R285[医药卫生—中药学] R363[医药卫生—中医学]