藏药紫金标抗HSV-1的作用机理研究  

Experimental study on the antiviral mechanism of Ceratostigma willmattianum against herpes simplex virus type 1 in vitro

作　　者：陈恬[1] 贾文祥[1] 杨发龙[1] 谢轶[1] 杨维青[1] 曾蔚[1] 张再容[1] 李晖 蒋思萍 央珍 陈金瑞 

机构地区：[1]四川大学华西基础医学与法医学院微生物学教研室,四川成都610041 [2]西藏高原生物研究所,西藏拉萨850002

出　　处：《中国中药杂志》2004年第9期882-886,共5页China Journal of Chinese Materia Medica

基　　金：西藏自治区科委重点项目 ( 2 0 0 3 12 4)

摘　　要：目的 :探讨紫金标抗单纯疱疹Ⅰ型病毒 (HSV 1)的作用及机理 ,为进一步开发该药提供理论依据。方法 :采用不同剂量的紫金标作用于适量HSV 1感染的Vero细胞 ,以 5 0 %组织细胞感染量 (TCID50 ) ,细胞病变效应(CPE) ,MTT法和核酸分子杂交作为评价指标。结果 :MTT法测得紫金标的 5 0 %抑制浓度 (IC50 )为 2 9.4 6mg·L-1,5 0 %中毒浓度 (TC50 )为 10 77mg·L-1,治疗指数 (TI)为 36 .5 6 ,结果表明紫金标有明显抑制HSV 1的作用 ,其作用强度和有效时间与药物浓度成正比。紫金标无直接灭活HSV 1的作用 ,也不能影响病毒的释放 ;但可干扰HSV 1对宿主细胞的吸附。不同浓度的紫金标能明显抑制HSV 1gD基因复制和mRNA表达。结论 :紫金标具有显著的抗HSV 1的作用 ,能抑制HSV 1对宿主细胞的吸附以及抑制HSVObjective: To study the antiviral effect a nd mechanisms of the liquid extract from Ceratostigma willmattianum against he rpes simplex virus type 1(HSV-1) in vitro. Method: C. w illmattianum in various concentration was applied to different steps of HSV -1 replication cycle. 50% Tissue culture infective dose (TCID 50 ), cytopat hic effect(CPE), MTT staining method, dot blotting and Northern blotting anal ysis were used to estimate index of antiviral activity. Result: 50% Toxic concentration (TC 50 ) was 1 077 mg·L -1 , IC 50 29.46 mg·L -1 and therapeutic index(TI) 36.56 in C. willmattianum. TC 50 330 mg·L -1 , 50% Inhibiting concentration (IC 50 ) 9.12 m g·L -1 and TI 36.18 in ACV by MTT staining method. The liquid extract fro m C. willmattianum had remarkable effect on inhibiting HSV-1 in vitro. Ce ratostigma could interfere absorption of HSV-1 to Vero cells to prevent HSV- 1 infectivity, inhibit HSV-1 gD DNA replication and HSV-1 gD mRNA expression. Conclusion: C. willmattianum possesses strong anti-HS V-1 activity in vitro. The antiviral mechanisms are related to inhibiting v irus absorption, HSV-1 gD gene replication and HSV-1 gD gene transcription. [

关 键 词：HSV-1 MTT法 藏药 感染 宿主细胞 抑制 作用强度 浓度 适量 作用机理 

分 类 号：R29[医药卫生—民族医学]