生物素抗生物素系统斑点免疫结合试验检测棘球蚴病患者的免疫应答  

BIOTIN-AVIDIN SYSTEM OF DOT-IMMUNOBINDING ASSAY IN THE DETECTION OF IMMUNE RESPONSE OF PATIENTS

作　　者：郑光宇[1] 赵荣乐[1] 

机构地区：[1]北京师范大学生命科学学院,北京100875

出　　处：《北京师范大学学报（自然科学版）》2004年第5期666-669,共4页Journal of Beijing Normal University(Natural Science)

基　　金：国家自然科学基金资助项目 (386 116 6 ) ;国家教育部留学回国人员科研基金资助项目

摘　　要：建立了生物素抗生物素系统斑点免疫结合试验 (BAS DIBA) ,并以此法研究了棘球蚴病患者对细粒棘球蚴 (Echinococcusgranulosus)抗原的免疫应答 .79例棘球蚴病患者血清、6 9例非棘球蚴病患者血清和 119例健康人血清的BAS DIBA试验结果为 :两试验检测抗棘球蚴抗原抗体的敏感性分别为 97.4 5 %和 92 .4 1% ;特异性分别为 98.4 0 %和 98.94 % ;在 91.14 %的棘球蚴病患者血清中 ,BAS DIBA试验较DIBA法敏感 ;两法同为阳性的棘球蚴病患者血清中 ,BAS DIBA检出的抗体效价的几何均数为DIBA法的 5 .4 8倍 ;4例DIBA试验阴性的棘球蚴病患者血清在BAS DIBA法中为阳性 .证实所建立的BAS DIBA法是检测棘球蚴病患者免疫应答敏感性高、特异性强的方法 ,特别是对于低抗体水平的棘球蚴病患者可能具有更高的应用价值 .Biotin-avidin system with dot-immunobinding assay(BAS-DIBA) is developed and is applied for the detection of immune response of anti-Echinococus granulosus antigens. In the series of 79 sera from clinically diagnosed cystic hydatidosis patients, 69 sera from non-hydatidosis patients and 119 sera from healthy blood donors, BAS-DIBA and standard DIBA have their respective sensitivity of 97.45% and 92.41% while the detecting specificity is 98.40% and 98.94% respectively; a higher sensitivity in BAS-DIBA than in standard DIBA with 4-8 folds increase in titer in 91.14% of hydatid sera; the geometric mean antibody titer detected with BAS-DIBA is 5.48 times of that detected with standard DIBA; 4 cases of hydatidosis with DIBA-negative result are positive by BAS-DIBA. The BAS-DIBA can provide a sensitive, specific procedure for detecting immune response of anti-Echinococcus granulosus antigens and may be of more applied value than the standard DIBA particularly in identification of the cases with lower immune response.

关 键 词：生物素-抗生物素系统 斑点免疫结合试验 棘球蚴病 免疫应答 

分 类 号：R383.33[医药卫生—医学寄生虫学] R446.61[医药卫生—基础医学]