大豆耐盐相关基因的分离及其功能鉴定  

Isolation and characterization of genes related to salt-tolerance in soybean

作　　者：余玉雯[1] 孙海丹[1] 郑易之[1] 兰英[1] 胥顺[1] 唐玉林[1] 麻晓亮[1] 

机构地区：[1]深圳大学生命科学学院深圳市微生物基因工程重点实验室,深圳518060

出　　处：《深圳大学学报（理工版）》2004年第4期324-330,共7页Journal of Shenzhen University(Science and Engineering)

基　　金：科技部国家转基因植物研究与产业化开发基金资助项目(JY03A18);教育部高等学校骨干教师资助项目(2000[65])

摘　　要：利用大肠杆菌功能表达体系,从大豆开花40d未成熟种子的cDNA表达文库中筛选获得了11个耐盐相关cDNA克隆.对其中3个克隆的插入片段进行测序.结果显示,其中的Gm1013为一尚未报道的新基因片段;GmRPS25 2编码的蛋白质序列与西红柿40S核糖体蛋白S25有91%的同源性;GmAIP 2编码的蛋白质序列与大豆根铝诱导蛋白SALI3 2有97%的同源性.其中GmAIP 2可表达分子量为30 8kD的多肽.pET GmAIP 2转化后的大肠杆菌在含800mmol/LNaCl和700mmol/LKCl的液体培养基中生长状况明显好于对照菌,即前者表现出较短的生长迟滞期和较高的生长量.11 salt-tolerant cDNA colonies were isolated from a cDNA library constructed from unmatured seeds of soybean by functional screening with E.coli.Sequencing of the inserts of 3 clolonies showed that Gm1013 is an unknown new gene; GmRPS25-2 is homologous to a gene encoding a 40S ribosomal protein S25 in tomato with 91% identity and GmAIP-2 is homologous to the gene encoding an aluminium-induced protein SALI3-2 in soybean with 97% identity. GmAIP-2 could express a protein of 30.8kD. The transformant containing pET-GmAIP-2 displayed enhanced salt tolerance in liquid mediums with 800 mmol/L NaCl or 700 mmol/L KCl when compared with the control strain, and showed the shorter lag period and higher bacterial quantity.

关 键 词：大肠杆菌 功能筛选 耐盐基因 大豆 

分 类 号：Q781[生物学—分子生物学]