糙皮侧耳菌漆酶的发酵及其对荨麻的应用  

Study on fermenting of pleurotus ostreatus laccase and application in the nettle deguming

作　　者：席丹[1] 陶用珍[1] 管映亭[2] 

机构地区：[1]西安交通大学生命学院,陕西西安710049 [2]西安工程科技学院纺织与材料学院,陕西西安710048

出　　处：《纺织高校基础科学学报》2004年第3期228-234,共7页Basic Sciences Journal of Textile Universities

摘　　要：通过对糙皮侧耳菌产漆酶发酵培养条件的优化,得到高效产漆酶的发酵培养条件(1L):麦草粉20g,酒石酸铵5.0g,pH5.0,吐温-800.1%,藜芦醇600μL,摇床转速为128r/min,温度30℃,微量元素液70mL.然后对发酵粗酶液进行初步分离纯化,经过超滤膜(Amincon)超滤浓缩,截留分子量为1.0×104的粗酶液,用初步纯化的漆酶液对荨麻进行生物脱胶,结果表明,漆酶对木质素去除率82%,残胶率降低了62%,比常规的化学脱胶去除木质素提高了20%.Optimizing pleurotus ostreatus'conditon of ferment culture, gained compositive culture which make the fungus the most efficienct producing laccase.Culture(1L)are made up of these factors:oaten power 20 g,tartaric acid ammonium 50 g,tween-80 0.1%,veratric alcohol 600μl,and distilled water,shake bed 128 r/min,temperature 30℃,pH 5.0.Afterwards, ferment fluid was separated, purified and ultrafiltrated(Am-(incon),Mr1.0×10~4). Finally, laccase was applied to the nettles for removal lignin .The better result was (obtained). Not only lignin was removed by 82%,but also remnant glue was reduced by 62%.The (removed) lignin by the purified laccase is 20% more than the removed lignin by conventional chemical reagent.

关 键 词：糙皮侧耳菌 漆酶 荨麻 木质素 

分 类 号：Q55[生物学—生物化学] O636.2[理学—高分子化学]