JAB1、LPS对糖皮质激素受体转录激活活性的影响  

Effects of JAB1 and LPS on glucocorticoid receptor transcription activation

作　　者：钟河江[1] 杨天德[1] 粟永萍[2] 

机构地区：[1]第三军医大学新桥医院麻醉科,重庆400037 [2]第三军医大学预防医学院全军复合伤研究所,重庆400038

出　　处：《重庆医学》2004年第11期1608-1610,共3页Chongqing medicine

基　　金：国家重点基础研究发展规划资助项目 ("973"项目 ) (G1 9990 542 0 1 )

摘　　要：目的　体外观察JAB1基因转染对糖皮质激素受体转录活性的调控作用。方法　采用体外培养COS 7细胞 ,脂质体法共转染含全长人JAB1基因质粒pCDNA3.1 JAB1、pCMV hGRα及含CAT报告基因质粒pMAMneo CAT ,设置不同剂量梯度的pCDNA3.1 JAB1质粒 ,转染 36h后用ELISA方法检测氯霉素乙酰转移酶 (CAT)活性来观察JAB1基因转染对GR转录激活活性的影响。结果　随着pCDNA3.1 JAB1质粒剂量的增加 ,CAT浓度也逐渐上升 ,表明GR的转录激活活性也逐渐增加。LPS能显著地降低CAT浓度 ,但共转染 pCDNA3.1 JAB1质粒可反转CAT活性的降低。结论　JAB1能增强GR的转录激活活性 ,LPS能抑制GR的转录激活能力。Objective To investigate the effects of JAB1 overexpression and LPS on glucocorticoid receptor transcription activation.Methods COS-7 cells were cultured in vitro, various doses of plasmid pCDNA3.1-JAB1 containing the full-length human cDNA encoding JAB1 were cotransfected with eukaryotic vector of pCMV-hGRα and pMAMneo-CAT by using the transfection reagent DOTAP, the expression of chloramphenicol acetyltransferase (CAT)was observed by CAT ELISA after the cotransfected COS-7 cells were cultured with dexamethasone for 36h. The ability of GR-mediated gene transcription was measured by using glucocorticoid response element (GRE) reporter plasmid pMAMneo-CAT analysis system.Results The concentration of CAT was enhanced with the increasing of doses of plasmid pcDNA3.1-JAB1. The production of CAT in cotransfected COS-7 cells was significantly decreased after the stimulation of LPS in present of dexamethasone, but reversed cotransfected with plasmid pcDNA3.1-JAB1.Conclusions Overexpression of JAB1 promoted the transcription activation of glucocorticoid receptor in vitro, LPS could inhibit the transcription activation of glucocorticoid receptor in vitro.

关 键 词：JAB1 糖皮质激素受体Α 氯霉素乙酰转移酶报告基因 LPS 

分 类 号：R341[医药卫生—基础医学]