烟雾吸入伤大鼠肺组织白介素-6和α-干扰素的变化及意义  

The changes and its significances of interleukin-6 and interferon-α of lung tissue in rats after smoke inhalation injury

作　　者：杨天德[1] 杨宗城[2] 罗奇志[2] 

机构地区：[1]第三军医大学新桥医院麻醉科,重庆400037 [2]第三军医大学西南医院烧伤研究所,重庆400038

出　　处：《重庆医学》2004年第11期1614-1615,共2页Chongqing medicine

基　　金：全军"九五"指令性课题 (96L0 4 3)

摘　　要：目的　探讨白介素 6 (IL 6 )和α 干扰素 (INF α)在烟雾吸入性肺损伤发生、发展中的作用。方法　建立大鼠烟雾吸入伤模型 ,将 6 0只Wistar大鼠随机分成正常对照组 ,烟雾吸入伤 1、3、6、12h和 2 4h组 ,分别取血和肺测定血气参数和肺组织含水量用干湿重法 ,肺血管通透性用13 1I 白蛋白渗出量测定 ,TNF α用放射免疫法测定 ,IL 6和INF α用ELISA法测定。结果　大鼠动脉血氧分压 (PaO2 )自烟雾吸入后 1h起均明显低于正常对照组 (P <0 .0 1) ,二氧化碳分压 (PaCO2 )仅烟雾吸入后 12h和 2 4h明显高于正常对照组 (P <0 .0 5 )。肺组织含水量和13 1I 白蛋白渗出量自烟雾吸入后 1h起均明显高于正常对照组 (P <0 .0 1) ,在伤后 6h达峰值 ;肺组织匀浆TNF α含量自烟雾吸入后 1h起至伤后 2 4h均明显高于正常对照组 (P <0 .0 1) ,IL 6含量自烟雾吸入后1h起至伤后 12h均明显高于正常对照组 (P <0 .0 1)、伤后 2 4h已有所恢复 ,INF α含量仅伤后 6h明显高于正常对照组 (P <0 .0 1)。结论　烟雾吸入后肺组织IL 6和INF α含量增加 ,可能参与了烟雾吸入性肺损伤的发生与发展 ,IL 6变化更早、持续时间更长。Objective To explore the changes and its significance of interleukin-6(IL-6) and interferon-α(INFα)of lung tissue in rats after smoke inhalation injury.Methods The model of smoke inhalation injury in rat was set up,sixty Wistar rats were randomly divided into six groups such as control group ,and the groups of 1h,3h,6h,12h and 24h following smoke inhalation.The lung tissue was isolated and the blood samples were get,and the data of blood gases and the water content of lung tissue were measured.The pulmonary vasopermeability was detected with 131 I-albumin exudation, TNF-α, IL-6 and INFαin lung tissue homogenate were assayed by radio-immunization and enzyme-linked immunosorbentassay(ELISA) respectively.Results Arterial PaO 2 from 1h to 24h following smoke inhalation were significantly lower than it in control group,but PaCO 2 in groups of 12h and 24h after smoke inhalation were markedly higher than it in control group.The water content and 131 I-albumin exudation of lung tissue from 1h to 24h following smoke inhalation were obviously increased while comparing with control group.TNF-αcontent in lung homogenate from 1h to 24h following smoke inhalation and IL-6 content in lung homogenate from 1h to 12h following smoke inhalation were remarkably higher than it in control group,and INF-α content in lung tissue homogenate only in 6h group after smoke inhalation was obviously higher than it in control group.Conclusion The increases in of IL-6 and INF-αin lung tissue after smoke inhalation should imply that IL-6 and INF-α take part in the generating and developing of smoke inhalation injury,and the action of IL-6 be more early and more persistent.

关 键 词：烟雾吸入伤 白介素-6 Α-干扰素 大鼠 

分 类 号：R363[医药卫生—病理学]