斑点杂交法检测SEN病毒感染  

Detection of SENV DNA in sera by dot blot

作　　者：杨群[1] 田德英[2] 许东[2] 葛娅[1] 任星峰[1] 宋佩辉[2] 

机构地区：[1]广州军区武汉总医院传染科,湖北武汉430070 [2]华中科技大学同济医学院附属同济医院传染科,湖北武汉430030

出　　处：《第四军医大学学报》2004年第20期1877-1879,共3页Journal of the Fourth Military Medical University

基　　金：湖北省科委资助项目 (2 0 0 2AA30 1C32 )

摘　　要：目的 :探讨斑点杂交法检测SEN病毒 (SENV)感染的应用价值 .方法 :应用地高辛标记、制备SENV部分基因探针并用斑点杂交技术检测SENVDNA ,与聚合酶链反应 (PCR)检测结果相比较 .结果 :在 191份血清中 ,采用斑点杂交法检出 6份阳性 ,检出率为 3.1% .PCR方法检测出 11份阳性 ,阳性率 5 .8% .结论 :制备的地高辛探针具有SENV特异性 ,以地高辛标记的SENV探针可用于检测SENV感染 .AIM: To explore the availability of dot-blot assay in the detection of SEN virus infection. METHODS: Nuclear acid was extracted from serum specimens, and dot-blot analysis and polymerase chain reaction(PCR) were carried out respectively to detect SENV DNA. RESULTS: Among 191 samples, 6 were found positive by dot-blot, and 11 were found positive by PCR. CONCLUSION: The probe prepared by us is SENV-specific. Dot-blot hybridization with DIG-labeled SENV DNA probe can be used to detect SENV DNA in sera samples.

关 键 词：斑点杂交法 SEN病毒感染 阳性 斑点杂交技术 针具 检出 地高辛标记 用地 PCR方法 聚合酶链反应(PCR) 

分 类 号：R512[医药卫生—内科学] R446[医药卫生—临床医学]