双生子人群β3AR Trp64Arg基因多态性与胰岛素敏感性的关系  

A twin study of association of β3AR Trp64Arg polymorphisms with insulin sensitivity

作　　者：李群娜[1] 詹思延[1] 吕筠[1] 秦颖[1] 郭晓霞[1] 曹卫华[1] 胡永华[1] 李立明[1] 

机构地区：[1]北京大学公共卫生学院流行病与卫生统计学系,北京100083

出　　处：《北京大学学报（医学版）》2004年第5期505-509,共5页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金 ( 3 0 0 70 672 );中华医学基金 ( 0 1 746)资助~~

摘　　要：目的 :探讨 β3肾上腺素能受体 (β3AR)Trp6 4Arg基因多态性与胰岛素敏感性的关系。 方法 :检测 88对异卵双生子 β3ARTrp6 4Arg基因多态性。用稳态模式评估法的对数 (logarithmtransformedhomeostasismodelassessment ,lgHOMA)来衡量胰岛素敏感性 ,分析β3ARTrp6 4Arg基因多态性与lgHOMA及体质指数 (bodymassindex ,BMI)的相关性。结果 :Trp6 4Trp、Trp6 4Arg和Arg6 4Arg的基因型频率分别为 71.5 % ,2 6 .7%和 1.7% ;有 β3ARTrp6 4Arg基因多态性的双生子胰岛素敏感性有降低的趋势 ,但是统计学差异无显著性 (P =0 .14 5 )。BMI与lgHOMA呈弱正相关 (r=0 .188,P =0 .0 0 2 ) ,β3ARTrp6 4Arg基因多态性与BMI的关联统计学差异无显著性 (P =0 .5 5 4 )。结论 :有 β3ARTrp6 4Arg基因多态性的双生子可能更容易导致胰岛素抵抗 ,但是这一结论还需扩大样本进行进一步的研究。Objective: To investigate the influences of β3 adrenergic receptor(β3AR) Trp64Arg polymorphism on insulin resistance. Methods: The insulin sensitivity was estimated with logarithm transformed homeostasis model assessment (HOMA). We enrolled 88 pairs of dizygotic twins for this study. We performed PCR-RFLP analysis to detect the Trp64Arg variants with BstO Ⅰ digestion. Results: The genotypic frequencies of Trp64Trp, Trp64Arg and Arg64Arg were 71.5%, 26.7% and 1.7%. Those who carried Trp64Arg variant appeared to have lower insulin sensitivity compared to the one who didn’t, but had no significance(P=0.145). Body mass index (BMI) had shown to be weakly positive correlated with lgHOMA. We found no significant association between the Trp64Arg variant and BMI. Conclusion: The presence of the Arg64 allele in β3AR gene may predispose patients to insulin resistance, and further study with larger sample size may help to confirm the results.

关 键 词：AR 胰岛素敏感性 双生子 多态性 BMI 人群 肾上腺素能受体 结论 对数 显著性 

分 类 号：R587[医药卫生—内分泌] R765[医药卫生—内科学]