用SSR标记检测同源四倍体与二倍体水稻的遗传差异  

GENETIC VARIATION OF TETRAPLOID AND DIPLOID RICE REVEALED BY MICROSATELLITE MARKS

作　　者：栾丽[1,2] 孔繁伦[1] 何涛[1] 刘刚[1] 涂升斌[1] 

机构地区：[1]中国科学院成都生物研究所 [2]中国科学院研究生院北京100039

出　　处：《应用与环境生物学报》2004年第5期556-558,共3页Chinese Journal of Applied and Environmental Biology

基　　金：四川省应用基础研究项目资助~~

摘　　要：随机选用分布于水稻 (OryzastativaL .) 12条染色体上的 15对SSR(simplesequencerepeats)引物 ,对 18种中科院成都生物所培育的四倍体水稻和 9种大面积种植的二倍体水稻进行了SSR多态性分析 .11对具多态性的引物共检测到 33条多态性条带 ,平均每对引物检测到 3个等位基因 .研究结果发现 ,所用同源四倍体水稻的基因组与二倍体水稻基因组大部分相同 ,只是在某些位点上具有差异 ,并筛选出部分SSR标记来区分二倍体与其同源四倍体 .本研究还对二倍体与同源四倍体之间遗传差异的原因进行了初步的探讨 .图 2表 2参Fifteen SSR (simple sequence repeats) primers, which disperse on 12 chromosomes in rice, were used to detect genetic diversity among eighteen tetraploid and nine diploid rice varieties. Those primers detected 33 alleles, and the average number of alleles per SSR locus was 3, with a range from 2 to 5. The result preliminary showed that most genomes of the tetraploid and diploid rice were the same except some sites .SSR could be used to differentiate diploid from tetraploid. Besides, the genetic difference between tetraploid and diploid rice was also studied. Fig 2, Tab 2, Ref 12

关 键 词：SSR 水稻 同源四倍体 

分 类 号：S511.032[农业科学—作物学]