VEGF-165在异位、在位和正常子宫内膜中的表达  

Expression of VEGF-165in Ectopic and Eutopic Endometrium from Women with and without Endometriosis

作　　者：沈育红[1] 唐中园[1] 喇端端[1] 

机构地区：[1]上海第二医科大学附属瑞金医院妇产科,200025

出　　处：《微循环技术杂志（临床与实验）》2004年第5期304-306,共3页

摘　　要：目的观察血管内皮生长因子 -165(VEGF -165)在异位、在位及正常子宫内膜中表达的特点 ,探讨其与子宫内膜异位症 (内异症 )的关系。方法应用免疫组化LSAB法和图像分析 ,检测VEGF -165蛋白在卵巢子宫内膜异位囊肿患者异位内膜26例、在位子宫内膜15例及正常子宫内膜20例中的表达特点及其阳性细胞的分布规律。结果VEGF -165在正常及异位子宫内膜腺上皮和基质细胞均有不同程度的表达 ,其中异位内膜组织中呈高表达状态,与正常子宫内膜和内异症在位内膜相比有明显差异(P<0.05) ;而VEGF-165于内异症在位内膜表达虽高于正常子宫内膜 ,但无统计学差异。结论VEGF-165在异位内膜组织中过度表达。Objective To evaluate the expression and localization of VEGF-165in ectopic and eutopic endometrium from women with and without endometriosis,thereby to determine the role of it in the pathogenesis of endometriosis.Methods The expression of VEGF-165protein on paraffin_embedded tissues was detected by imˉmunohistochemical analysis _LSAB and image analysis.The study included endometriotic tissues(n=26)and matched eutopic endometrium(n=15)of patients with endometriosis and eutopic endometrium of normal menstruatˉing women without endometriosis(n=20).Results The variant expression of VEGF-165was detected in both eutopic and ectopic endometrium.Ectopic tissues showed statistically stronger VEGF-165protein compared with eutopic endometrium from normal and endometriosis patients(P<0.05).However,we did not find their different expression between eutopic endometrium of women with endometriosis and normal controls.Conclusions Ectopic endometrium displayed greater VEGF-165expression.These results suggest that VEGF play an important role in the pathogenesis of the disease.

关 键 词：正常 VEGF 子宫内膜异位症 表达 内异症 异位内膜 在位内膜 血管内皮生长因子 LSAB法 异位子宫内膜 

分 类 号：R711.71[医药卫生—妇产科学] R735[医药卫生—临床医学]