层粘连蛋白、纤粘连蛋白、IV型胶原在人月经周期子宫内膜中的表达  

Expression of laminin, fibronectin and collagen IV in normal human endometrium during menstrual cycle

作　　者：马彩虹[1] 陈贵安[1] 王海燕[1] 高冬霞[2] 

机构地区：[1]北京大学第三医院生殖医学中心,北京100083 [2]北京大学第三医院生殖医学中心病理科,北京100083

出　　处：《生殖医学杂志》2004年第5期257-261,共5页Journal of Reproductive Medicine

摘　　要：目的　研究细胞外基质蛋白层粘连蛋白 (LN)、纤粘连蛋白 (FN)和IV型胶原 (IVCL)在正常人月经周期子宫内膜中的表达。　方法　采用免疫组织化学方法检测 4 9例正常月经周期子宫内膜中LN、FN、IVCL蛋白的表达。　结果　LN主要分布于腺上皮及腔上皮基底膜 ,分泌中期表达最强。FN主要分布于间质 ,腔上皮及腺上皮基底膜也均有表达 ,分泌中期含量丰富。IVCL在腺上皮基底膜的表达分泌中期最强 ,在间质的表达分泌早期最强 ,分泌中、晚期表达逐渐减弱。LN、FN、IVCL在血管基底膜上均有表达 ,无周期性变化。　结论　LN、FN、IVCL在月经周期子宫内膜中的表达随月经周期而变化。Objective: To study the expression of laminin, fibronectin and collagen IV in normal human endometrium during menstrual cycle. Method:Immunohistochemistry was used to measure the expression of laminin, fibronectin and collagen IV in normal endometrium of 49 women. Results: Laminin immunostaining was found in the basement membrane of surface epithelium and glandular endometrium. The expression in mid-secretory phase was significantly higher. Fibronectin was primarily present throughout the stroma and also in the basement membrane of surface epithelium and glandular endometrium. The expression in mid-secretory phase was higher significantly. Collagen IV was localized in the basement membrane of glandular epithelium and stroma. The expression in glandular epithelium was the highest in mid-secretory phase, while the expression in stroma peaked in early-secretory phase and gradually decreased afterwarsds. The expression of laminin, fibronectin and collagen IV in the basement membrane of blood vessel was found with no significant changes during the menstrual cycle. Conclusion: Laminin, fibronectin and collagen IV showed a pronounced cycle-dependent expression in cycling endometrium.

关 键 词：子宫内膜 蛋白层粘连蛋白 纤粘连蛋白 Ⅳ型胶原 

分 类 号：R711.74[医药卫生—妇产科学]